NHLBI MINORITY SCHOOL DEVELOPMENT AWARD

NHLBI 少数族裔学校发展奖

• 批准号: 3088422
• 负责人: STEVEN A EDWARDS
• 金额: $ 4.84万
• 依托单位: MEHARRY MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-07-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3088422
• 关键词: gene expression; minority institution research support; nucleic acid sequence; oligonucleotides; posttranslational modifications; regulatory gene; transcription factor

Egr-l, EGR2, and EGR3 are early response genes, meaning that they are rapidly and transiently stimulated by mitogens, growth factors, and a variety of other stimuli. They are also zinc finger containing transcription factors which bind specifically to the sequence GCGGGGCG, a specificity which is shared by two related factors, NGF1-C, and the product of the Wilm's tumor locus, WT33. There is evidence to suggest that egr-l is involved in establishing and maintaining differentiated function in cardiac muscle and I neural cell types. The goals of this research project are: 1) To determine whether the expression of egr-like transcription factors are critical to growth control and differentiation and to identify potential target genes. 2) To determine the function of particular protein domains of egr-l. 3) To characterize and determine the function of post-translational modifications of egr-l. Results obtained in these experiments should help elucidate how differentiation pathways are determined and how differentiated function of certain tissues is maintained. This research should also lead to a greater understanding of the egr family of transcriptional regulators. If awarded, the proposed grant will support and maintain a research effort at Meharry Medical College focused on the field of transcriptional regulation. This will broaden the available opportunities for training minority students in the Biochemistry Department. The formal relationship proposed with Dr. Daryl Granner of Vanderbilt University will afford reciprocal training opportunities and technology transfer between the two schools.

Egr-1、EGR2 和 EGR3 是早期反应基因，这意味着它们 受到有丝分裂原、生长因子和 各种其他刺激。 它们还含有锌指 与序列 GCGGGGCG 特异性结合的转录因子， 两个相关因子 NGF1-C 和 维尔姆氏肿瘤基因座 WT33 的产物。 有证据表明 egr-l 参与建立和维持差异化 在心肌和神经细胞类型中发挥功能。 本研究项目的目标是： 1) 确定是否 EGR样转录因子的表达对于生长至关重要 控制和分化并识别潜在的靶基因。 2) 至 确定egr-1特定蛋白结构域的功能。 3) 至 表征并确定翻译后功能 egr-l的修改。 这些实验中获得的结果应该 帮助阐明分化途径是如何确定的以及如何 某些组织的分化功能得以维持。 这项研究 还应该导致人们对 EGR 系列有更深入的了解 转录调节因子。 如果获得资助，拟议的拨款将支持和维持一项研究 梅哈里医学院的工作重点是转录领域 规定。 这将扩大可用的培训机会 生物化学系的少数民族学生。 正式的 与范德堡大学 Daryl Granner 博士建立关系 将提供互惠培训机会和技术转让 两所学校之间。