GENETIC STUDIES OF CAMPYLOBACTER RECTUS

直弯杆菌的遗传学研究 厌氧革兰氏阴性菌活动杆中的直弯曲杆菌 其通常分离自成人牙周炎损伤， 牙周炎病变的患者发现全身性疾病， 如糖尿病和获得性免疫缺陷综合症。而 横截面研究暗示这个物种是一个重要的 牙周致病菌中，对C.直肌 确定毒力因子将是一个重要步骤， 阐明这种生物体在发病机制中的作用， 牙周病C.直肌 是一种杀死人类多形性白细胞的毒素， 早幼粒细胞HL-60细胞，并可能允许C.直肌克服 宿主的防御和定植牙周组织。 这些研究的长期目标是获得更好的 理解C的作用。直肌与牙周病 和口腔外感染，然后制定策略， 控制和预防C.直肌相关性牙周炎 具体而言，我们建议：（1）检查遗传学和血清学 C.内部异质性（2）将毒素基因克隆到E. （3）确定结构和转录组织 负责毒素产生的一个或多个基因，以及;（4） 研究毒素基因的结构/功能关系 产品 关键词：直弯杆菌，毒素，克隆

Genetic Studies of Campylobacter rectus Campylobacter rectus in an anaerobic Gram negative, motile rod which is often isolated from adult periodontitis lesions including periodontitis lesions found in patients with systemic diseases such as diabetes mellitus and Acquired Immunodeficiency Syndrome. While cross-sectional studies implicate this species as an important periodontal pathogen, relatively little is know about C. rectus. Identification of virulence factors would be an important step in elucidating the role this organism plays in the pathogenesis of periodontal disease. One potential virulence factor from C. rectus is a toxin which kills human polymorphonuclear leukocytes and promyelocytic HL-60 cells and which may allow C. rectus to overcome host defenses and to colonize the periodontium. The long term objective of these studies is to gain a better understanding of the role of C. rectus in human periodontal disease and in extraoral infections and then to develop strategies for the control and prevention of C. rectus associated periodontitis. Specifically we propose to; (1) examine genetic and serologic heterogeneity within C. rectus, (2) clone the toxin gene into E. Coli; (3) determine the structure and transcriptional organization of the gene or genes responsible for the toxin production, and; (4) study the structure/function relationships of the toxin gene product. Key Words: Campylobacter rectus, toxin, cloning