TRANSEPITHELIAL TRANSPORT IN CULTURED UTERINE CELLS

描述(改编自申请人的摘要)：这第二个 对R29申请的修订旨在促进我们对 水在人体内运动的分子机制 宫颈上皮屏障。申请者将使用培养的人类 他的实验室开发了上皮细胞模型，以探索三种 假设：1)雌激素通过以下途径增加宫颈通透性 对宫颈细胞紧密连接的影响方式，2)雌激素 增加细胞内的钙动员，进而增加细胞内的 细胞间隙的通透性；3)细胞间隙的通透性 人宫颈细胞内钙离子的动员是钙依赖的 刺激细胞的KCl运输，从而导致减少 细胞体积。建议将这些研究的结果 加深我们对宫颈积液基本生物学的了解 动态平衡，但也更好地理解临床相关 宫颈液体状态异常的设置。

DESCRIPTION (Adapted from the applicant's abstract): This second revision of an R29 application seeks to advance our understanding of the molecular mechanisms by which the movement of water across the human cervical epithelial barrier. The applicant will use cultured human epithelial cell models developed in his laboratory to explore three hypotheses: 1) that estrogen increases transcervical permeability by means of an effect on cervical cell tight junctions, 2) that estrogen increases calcium mobilization in the cells, which in turn increases the permeability of the intercellular space, and 3) that the mechanism of Ca++ mobilization in the human cervical cell is a Ca++-dependent stimulation of cellular KCl transport, which results in a reduction in cell volume. It is proposed that the results of these studies will increase both our understanding of the basic biology of cervical fluid homeostasis, but also a better understanding of clinically relevant settings in which the cervical fluid status is abnormal.