TRANSEPITHELIAL TRANSPORT IN CULTURED UTERINE CELLS

描述（改编自申请人的摘要）：第二次 R29应用程序的修订旨在促进我们对 水在人体内流动的分子机制 宫颈上皮屏障申请人将使用培养的人类 在他的实验室中开发的上皮细胞模型， 假设：1）雌激素通过以下方式增加跨膜渗透性： 影响宫颈细胞紧密连接的手段，2）雌激素 增加了细胞中钙的动员，这反过来又增加了 细胞间隙的渗透性，和3）的机制， 人宫颈细胞中的Ca++动员是Ca++依赖性的 刺激细胞KCl转运，导致 细胞体积建议这些研究的结果将 增加我们对宫颈液的基本生物学的理解， 稳态，而且更好地了解临床相关的 宫颈液状态异常的情况。

DESCRIPTION (Adapted from the applicant's abstract): This second revision of an R29 application seeks to advance our understanding of the molecular mechanisms by which the movement of water across the human cervical epithelial barrier. The applicant will use cultured human epithelial cell models developed in his laboratory to explore three hypotheses: 1) that estrogen increases transcervical permeability by means of an effect on cervical cell tight junctions, 2) that estrogen increases calcium mobilization in the cells, which in turn increases the permeability of the intercellular space, and 3) that the mechanism of Ca++ mobilization in the human cervical cell is a Ca++-dependent stimulation of cellular KCl transport, which results in a reduction in cell volume. It is proposed that the results of these studies will increase both our understanding of the basic biology of cervical fluid homeostasis, but also a better understanding of clinically relevant settings in which the cervical fluid status is abnormal.