TRANSEPITHELIAL TRANSPORT IN CULTURED UTERINE CELLS

培养的子宫细胞中的跨上皮运输

• 批准号: 2889073
• 负责人: GEORGE I GORODESKI
• 金额: $ 10.71万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2889073
• 关键词: adenosine triphosphate; biological fluid transport; calcium flux; cell morphology; cervix; chloride channels; diethylstilbestrol; electrical conductance; electrochemistry; epithelium; estradiol; estrogen inhibitor; estrogens; hormone regulation /control mechanism; human tissue; intracellular transport; membrane permeability; potassium channel; potassium chloride; progesterone; tamoxifen; tight junctions; tissue /cell culture

DESCRIPTION (Adapted from the applicant's abstract): This second revision of an R29 application seeks to advance our understanding of the molecular mechanisms by which the movement of water across the human cervical epithelial barrier. The applicant will use cultured human epithelial cell models developed in his laboratory to explore three hypotheses: 1) that estrogen increases transcervical permeability by means of an effect on cervical cell tight junctions, 2) that estrogen increases calcium mobilization in the cells, which in turn increases the permeability of the intercellular space, and 3) that the mechanism of Ca++ mobilization in the human cervical cell is a Ca++-dependent stimulation of cellular KCl transport, which results in a reduction in cell volume. It is proposed that the results of these studies will increase both our understanding of the basic biology of cervical fluid homeostasis, but also a better understanding of clinically relevant settings in which the cervical fluid status is abnormal.

描述（改编自申请人的摘要）：第二次 R29应用程序的修订旨在促进我们对 水在人体内流动的分子机制 宫颈上皮屏障申请人将使用培养的人类 他的实验室开发了上皮细胞模型来探索三种 假设：1）雌激素通过以下方式增加跨膜渗透性： 影响宫颈细胞紧密连接的手段，2）雌激素 增加了细胞中钙的动员，这反过来又增加了 细胞间隙的渗透性，和3）的机制， 人宫颈细胞中的Ca++动员是Ca++依赖性的 刺激细胞KCl转运，导致 细胞体积建议这些研究的结果将 增加我们对宫颈液的基本生物学的理解， 稳态，而且更好地了解临床相关的 宫颈液状态异常的情况。

项目成果

Estrogen abrogates transcervical tight junctional resistance by acceleration of occludin modulation.

• DOI: 10.1210/jc.2004-0823
• 发表时间: 2004-10
• 期刊: The Journal of clinical endocrinology and metabolism
• 作者: R. Zeng;Xin Li;G. Gorodeski

Apically sorted P2X7 receptors mediate purinergic-induced pore formation preferentially in apical domains of the plasma membrane.

顶端分选的 P2X7 受体优先在质膜的顶端区域介导嘌呤能诱导的孔形成。

• DOI: 10.1080/15257770600890913
• 发表时间: 2006
• 期刊: Nucleosides, nucleotides & nucleic acids
• 作者: Li,X;Gorodeski,GI
• 通讯作者: Gorodeski,GI

Antiapoptotic effects of estrogen in normal and cancer human cervical epithelial cells.

雌激素对正常和癌症人宫颈上皮细胞的抗凋亡作用。

• DOI: 10.1210/en.2004-0807
• 发表时间: 2004
• 期刊: Endocrinology
• 影响因子: 4.8
• 作者: Wang,Qifang;Li,Xin;Wang,Liqin;Feng,Ying-Hong;Zeng,Robin;Gorodeski,George
• 通讯作者: Gorodeski,George

Estrogen modulation of MgATPase activity of nonmuscle myosin-II-B filaments.

雌激素对非肌肉肌球蛋白-II-B 丝的 MgATP 酶活性的调节。

• DOI: 10.1210/en.2006-1045
• 发表时间: 2007
• 期刊: Endocrinology
• 影响因子: 4.8
• 作者: Gorodeski,GeorgeI

P2X7 receptor-mediated apoptosis of human cervical epithelial cells.

• DOI: 10.1152/ajpcell.00256.2004
• 发表时间: 2004-11
• 期刊: American journal of physiology. Cell physiology
• 作者: Qifang Wang;Liqin Wang;Ying-hong Feng;Xin Li;R. Zeng;G. Gorodeski