Industrial Platform Development for Commercial Enzyme Production

商业酶生产工业平台开发

• 批准号: 131910
• 金额: $ 16.47万
• 依托单位: BIOCATALYSTS LIMITED
• 依托单位国家: 英国
• 项目类别: Feasibility Studies
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=131910
• 关键词: Industrial; Platform; Development; Commercial; Enzyme

Enzymes are biological molecules that facilitate chemical reactions in living cells. Many products in the food, fine chemical, flavour & fragrance, pharmaceutical and biotherapeutic industries use enzymes in their manufacturing processes. Many enzymes on the market are isolated from their original wild-type organism, many more need to be produced in a different host organism that is more suitable for large-scale industrial production and is capable of providing commercially viable yields of the enzyme. To optimise the level of production of the enzyme is time consuming and costly and often results in failure to achieve commercial yield targets due to the inherent biology of the host and the enzyme. Therefore, this project will develop a broad-host range expression system for expressing any new enzyme in a selection of industrially compatible microorganisms and assessing enzyme production in these multiple hosts prior to selecting the one that provides the highest yields for that enzyme.

酶是促进活细胞中化学反应的生物分子。食品、精细化工、香料和香料、制药和生物治疗行业的许多产品在其制造过程中使用酶。市场上的许多酶都是从它们原来的野生型生物体中分离出来的，更多的酶需要在更适合大规模工业生产并能够提供商业上可行的酶产量的不同宿主生物体中生产。优化酶的生产水平既耗时又昂贵，而且由于宿主和酶的固有生物学特性，往往导致无法实现商业产量目标。因此，该项目将开发一个广泛的宿主范围的表达系统，用于在一系列工业兼容的微生物中表达任何新的酶，并在选择提供该酶产量最高的一个之前评估这些多个宿主中的酶产量。