Maximising synthetic peptide and protein manufacture by in vivo DNA assembly in bacteria using high throughput robotics

使用高通量机器人技术通过细菌体内 DNA 组装最大限度地生产合成肽和蛋白质

• 批准号: 131917
• 金额: $ 23.79万
• 依托单位: OXFORD GENETICS LTD
• 依托单位国家: 英国
• 项目类别: Feasibility Studies
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=131917
• 关键词: Maximising; synthetic; peptide; protein; manufacture

Many different factors influence whether a piece of DNA will work in a biological setting, to express the protein it encodes. Proteins represent a broad new class of exciting but expensive new medicines. Poor DNA activity is particularly problematic when manufacturers of proteins need to produce large quantities by industrial manufacture. At Oxford Genetics we have developed a wide range of tools and expertise to allow us to design and build DNA sequences that produce high amounts of proteins. In this project we aim to industrialise a large proportion of our existing work flow and make the assembly of complex DNA an automated high-throughput process. This will enable the rapid and efficient development of DNA sequences that are optimal for producing proteins in any system, and should lead to major improvements in protein manufacture. This will benefit many aspects of commerce and medicine in the UK.

许多不同的因素会影响一段DNA是否会在生物环境中工作，以表达它编码的蛋白质。蛋白质代表了一个令人兴奋但昂贵的新药物的广泛的新类别。当蛋白质制造商需要通过工业生产大量生产时，DNA活性差是特别成问题的。在牛津遗传学，我们开发了广泛的工具和专业知识，使我们能够设计和构建产生大量蛋白质的DNA序列。在这个项目中，我们的目标是将我们现有的大部分工作流程工业化，并使复杂DNA的组装成为一个自动化的高通量过程。这将使DNA序列的快速和有效的发展是最佳的生产蛋白质在任何系统中，并应导致重大改进蛋白质的生产。这将有利于英国商业和医学的许多方面。