HIGH RESOLUTION MASS SPECTRA OF BIOORGANIC COMPOUNDS

生物有机化合物的高分辨率质谱

• 批准号: 2174871
• 负责人: KENNETH L RINEHART
• 金额: $ 18.03万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-05-01 至 1996-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2174871
• 关键词: Archaea; Haemophilus influenzae; antibiotics; antifungal antibiotics; antineoplastics; antiviral agents; biomedical equipment development; cardiotoxin; chlorin; cytochrome P450; enzyme structure; estrogen receptors; gamma aminobutyrate; glycolipids; glycosphingolipids; hemoprotein; hemoprotein structure; high performance liquid chromatography; hydroxamate; ionization; mass spectrometry; metalloporphyrins; neuropeptides; neurotoxins; oxidoreductase; peptide structure; phospholipids; polyenes; protein structure; steroids; trehalose; virus protein

The overall objective of the proposed research continues to be to develop and use mass spectrometry as a tool for solving problems of biomedical interest, especially those dealing with the characterization of complex polar compounds and others of high molecular weight. The powerful research mass spectrometers--a VG 70-SE4F and a VG ZAB-SE--will be used in assigning structures to complex peptides and proteins: aldose reductases, cytochromes, marine-derived toxins, insect and marine invertebrate neuropeptides, marine algal and cyanobacterial GABA-mimetic peptides, and peptide antibiotics. The instruments will also be employed in improving or developing the FAB technique: by improving high-resolution FAB; by enhancing ion production with laser assistance; developing LC/HRMS and LC/MS/MS; and by applying LC/FAB to gradient elution HPLC and countercurrent chromatography (CCC). The mass spectrometric techniques developed will be applied to solving structural problems of the P.I. (antiviral and antitumor agents from marine sources, antibiotics, marine-derived chlorins, cyanobacterial toxins, a hibernation factor in bears' blood, hydroxamates from Hemophilus influenzae) and other biomedical scientists at the University of Illinois (i.a., methanogen cofactors, metallo-porphyrins, estrogen receptors, fluorinated steroids, viral proteins, phospholipids, glycolipids, and glycosphingolipids).

拟议研究的总体目标是发展 并使用质谱法作为解决生物医学问题的工具 兴趣，尤其是那些处理复杂表征的人 极性化合物和其他具有高分子量的化合物。 强大的研究质谱仪 - VG 70-SE4F和VG zab-se-将用于将结构分配给复杂的肽和 蛋白质：醛糖还原酶，细胞色素，海洋衍生的毒素，昆虫 和海洋无脊椎动物神经肽，海洋藻类和蓝细菌 GABA模拟肽和肽抗生素。 乐器也将 用于改进或开发Fab技术：通过改进 高分辨率晶圆厂；通过激光辅助增强离子生产； 开发LC/HRM和LC/MS/MS；并通过将LC/Fab应用于梯度洗脱 HPLC和反电色谱法（CCC）。 开发的质谱技术将用于解决 P.I.的结构问题（来自海洋的抗病毒和抗肿瘤剂 来源，抗生素，海洋衍生的氯蛋白，蓝细菌毒素，一种 熊的血液中的冬眠因子，血友病的羟胺 伊利诺伊大学的流感）和其他生物医学科学家 （I.A.，甲烷基辅因子，金属孢子蛋白，雌激素受体， 氟化类固醇，病毒蛋白，磷脂，糖脂和 糖磷脂）。

项目成果

A trithiolate tripodal bifunctional ligand for the radiolabeling of peptides with gallium(III).

一种三硫醇三足双功能配体，用于用镓 (III) 放射性标记肽。

• DOI: 10.1021/bc025552g
• 发表时间: 2002
• 期刊: Bioconjugate chemistry
• 影响因子: 4.7
• 作者: Luyt,LeonardG;Katzenellenbogen,JohnA
• 通讯作者: Katzenellenbogen,JohnA

Primary sequence analysis by fast atom bombardment mass spectrometry of a peptide with adipokinetic activity from the corpora cardiaca of the cricket Gryllus bimaculatus.

通过快原子轰击质谱法对来自蟋蟀双斑蝼蛄心脏体的具有脂肪运动活性的肽进行一级序列分析。

• DOI: 10.1016/0006-291x(87)90494-3
• 发表时间: 1987
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Gäde,G;Rinehart,KL
• 通讯作者: Rinehart,KL

1,2-bis(4-Hydroxyphenyl)-3,4-dihydro-6-hydroxynaphthalene, a photofluorogenic ligand for the estrogen receptor.

1,2-双(4-羟基苯基)-3,4-二氢-6-羟基萘，雌激素受体的光致荧光配体。

• DOI: 10.1111/j.1751-1097.1986.tb09502.x
• 发表时间: 1986
• 期刊: Photochemistry and photobiology
• 影响因子: 3.3
• 作者: Bindal,RD;Katzenellenbogen,JA
• 通讯作者: Katzenellenbogen,JA

Microbial transformations of natural antitumor agents. 13. Conversions of 13-blocked anthracyclinones.

天然抗肿瘤剂的微生物转化。

• DOI: 10.7164/antibiotics.33.705
• 发表时间: 1980
• 期刊: The Journal of antibiotics
• 作者: Wu,GS;Gard,A;Rosazza,JP
• 通讯作者: Rosazza,JP

Diatomic monocations and dications containing germanium and a rare gas atom.

含有锗和稀有气体原子的双原子单阳离子和双阳离子。

• DOI: 10.1002/rcm.1290050203
• 发表时间: 1991
• 期刊: Rapid communications in mass spectrometry : RCM
• 作者: Curtis,JM;Rong,LS
• 通讯作者: Rong,LS