Cell-Sort MultiTool: a Novel Platform for Bacterial Single-Cell Analysis

该项目旨在利用一种集成了微流体、显微镜和基因组学的创新方法，在单细胞水平上促进对细菌群体中抗生素耐药性的分子理解。对单个细菌细胞遗传学的研究对于提供对异质性耐药性的基本见解至关重要，异质性耐药性是耐药性发展的重要组成部分。预期结果包括高通量多路筛选的新平台技术，以及改善对细菌异质性的了解，从而告知抗生素的使用。这个跨学科的项目通过降低医疗成本、促进澳大利亚人的健康并将先进技术商业化，应该会产生显著的社会和经济效益。

This project aims to advance molecular understanding of antibiotic resistance in bacterial populations at the single-cell level, using an innovative approach integrating microfluidics, microscopy and genomics. The study of individual bacterial cell genetics is essential to provide fundamental insights into heterogeneous resistance, an important component of resistance development. Expected outcomes include a new platform technology for high-throughput multiplexed screening and improved knowledge of bacterial heterogeneity, informing antibiotic usage. This interdisciplinary project should yield significant benefits in society and economy by reducing healthcare costs, boosting health for Australians and commercialising advanced technologies.