BIOLOGY OF THE P53 PROTEIN

增殖细胞核抗原（PCNA）作为DNA的辅助因子， 聚合酶δ是DNA复制和细胞增殖所必需的。 增殖 PCNA的表达与其他基因的表达一样， 编码DNA合成装置的蛋白质， 血清或生长因子刺激的Go细胞中的G1/S边界。 的 这些基因的协同表达表明，尽管一些 可能存在多样性，但也必须有一些共同的机制 几乎同时被激活 通过研究 PCNA表达，我们希望确定调控蛋白， 调节PCNA表达（本身具有相当大的意义），但可能 也参与了其他G1/S边界基因的调控。 以来 G1/S转换是细胞调控的关键步骤之一 增殖，在这一点上，调节蛋白的鉴定是 意义重大

The Proliferating Cell Nuclear Antigen (PCNA), as the co-factor of DNA polymerase delta, is absolutely required for DNA replication and cellular proliferation. The expression of PCNA, like the expression of other genes coding for proteins of the DNA synthesizing apparatus, increases sharply at the G1/S boundary in Go cells stimulated by serum or growth factors. The co-ordinate expression of these genes indicates that, although some diversity may be present, some common mechanism(s) must also be operative in their near-simultaneous activation. By investigating the regulation of PCNA expression, we hope to identify regulatory proteins that not only modulate PCNA expression (in itself of considerable interest) but may be also involved in the regulation of other G1/S boundary genes. Since the G1/S transition is one of the critical steps in the control of cell proliferation, the identification of regulatory proteins at this point is of major significance.