BIOLOGY OF THE P53 PROTEIN

P53 蛋白的生物学

• 批准号: 2177098
• 负责人: RENATO Luigi BASERGA
• 金额: $ 44.3万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-01 至 1996-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2177098
• 关键词: DNA binding protein; DNA replication; binding proteins; cell cycle proteins; cell growth regulation; complementary DNA; gel electrophoresis; gene expression; genetic mapping; genetic promoter element; messenger RNA; microinjections; molecular cloning; molecular genetics; northern blottings; nucleic acid sequence; polymerase chain reaction; proliferating cell nuclear antigen; protein biosynthesis; protein purification; protein sequence; structural genes; tissue /cell culture; transcription factor; transfection

The Proliferating Cell Nuclear Antigen (PCNA), as the co-factor of DNA polymerase delta, is absolutely required for DNA replication and cellular proliferation. The expression of PCNA, like the expression of other genes coding for proteins of the DNA synthesizing apparatus, increases sharply at the G1/S boundary in Go cells stimulated by serum or growth factors. The co-ordinate expression of these genes indicates that, although some diversity may be present, some common mechanism(s) must also be operative in their near-simultaneous activation. By investigating the regulation of PCNA expression, we hope to identify regulatory proteins that not only modulate PCNA expression (in itself of considerable interest) but may be also involved in the regulation of other G1/S boundary genes. Since the G1/S transition is one of the critical steps in the control of cell proliferation, the identification of regulatory proteins at this point is of major significance.

增殖细胞核抗原 (PCNA)，作为 DNA 的辅助因子 聚合酶 δ 是 DNA 复制和细胞绝对必需的 增殖。 PCNA的表达，与其他基因的表达一样 编码 DNA 合成装置的蛋白质，在 血清或生长因子刺激的 Go 细胞中的 G1/S 边界。 这 这些基因的协调表达表明，尽管有些基因 可能存在多样性，一些共同机制也必须发挥作用 它们几乎同时激活。 通过调查监管 PCNA 表达，我们希望鉴定出调节蛋白，不仅 调节 PCNA 表达（本身就很有趣），但可能 还参与其他G1/S边界基因的调节。 自从 G1/S转变是细胞控制的关键步骤之一 增殖，此时调节蛋白的鉴定是 具有重大意义。