NONDIAMAGNETIC AGENTS IN IN VIVO 23NA AND 1H20 MR

非抗磁剂体内 23NA 和 1H20 MR

• 批准号: 2176446
• 负责人: CHARLES S. SPRINGER
• 金额: $ 13.27万
• 依托单位: ASSOCIATED UNIV-BROOKHAVEN NATL LAB
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-04-01 至 1997-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2176446
• 关键词: Rana; alternatives to animals in research; bioengineering /biomedical engineering; biomagnetism; biophysics; blood brain barrier; cations; computer program /software; contrast media; image processing; ion transport; ionophores; laboratory mouse; laboratory rat; magnetic resonance imaging; mathematical model; neuroanatomy; nuclear magnetic resonance spectroscopy; phantom model; pharmacokinetics; relaxation spectrometry; sodium; sodium channel; toad; water; yeasts

The work proposed here will continue to explore and demonstrate the range of applications of the effects of non-diamagnetic agents on the two strongest tissue NMR signals: those of 1H2O and 23Naaq. Specific projects in two general categories are proposed. It will be demonstrated that one can make 1H2O MR images of contrast reagent (CR) distribution volumes directly. This is a very important goal that has not yet been achieved in MRI. It is fundamental, and of utmost significance to more sophisticated applications of CRs. The new technique of combined relaxography and imaging (CRI), recently introduced by this laboratory, is key to the production of distribution volume images. It is explained in this proposal. Studies of cell suspension phantoms, mice, and rats are proposed to demonstrate this approach in vivo. The CRI technique is totally general and applicable to longitudinal, transverse, or rotating-frame NMR relaxation and can be used to study any CR, whether employing the hyperfine or the bulk magnetic susceptibility mechanisms (or both). Longitudinal relaxation and hyperfine CRs are emphasized in this proposal. The yeast cell suspension and rodent studies proposed will also allow the quantitative determination of the extent of compartmental H2O exchange and should permit and production of exchange maps in vivo. The rodent experiments will allow very quantitative measurements of changes in brain CR distribution volumes upon transient disruption of the blood-brain-barrier by non-lethal hyperosmolar intracarotid infusion. Completely analogous 23Na MR studies are proposed. These will employ spectroscopic imaging techniques to make direct distribution volume images of shift reagents (SRs). The main differences expected include the following. Compartmental Na+ exchange is extremely slow and will have no effect on the 23Na spectroscopic images. The electronic charges of the in vivo SR anions are greater than those of the CR anions. Thus, it will be interesting to see if the distribution volumes of the former are at all restricted when compared with those of the latter. This work involves aspects of physics, physical and synthetic chemistry, biophysics, and physiology and has ramifications in the study of a number of pathological conditions including neurological and cardiovascular disorders.

这里提出的工作将继续探索和展示范围 非抗磁剂对两种药物作用的应用 最强的组织NMR信号：1H2O和23Naaq的那些。 具体 拟议的项目分为两大类。 它将被证明，人们可以使1H2O磁共振图像的对比度 试剂（CR）分布体积直接。 这是一个非常重要 这是MRI尚未实现的目标。 这是基本的， 这对CR的更复杂应用具有重要意义。 新 最近引入的联合弛豫成像技术（CRI） 通过这个实验室，是关键的生产分布量 图像. 这一点在本提案中有说明。 细胞悬液的研究 幻影，小鼠和大鼠被提出来证明这种方法， vivo. CRI技术是完全通用的，适用于 纵向、横向或双标架NMR弛豫，并且可以是 用于研究任何CR，无论是采用超精细还是散装 磁化率机制（或两者）。 纵向弛豫 和超精细CR在这个建议中被强调。 所述酵母细胞 悬浮液和啮齿动物研究建议也将允许定量 确定隔室H2O交换的程度，并应 允许和生产的交换地图在体内。 啮齿动物实验 将允许非常定量地测量大脑CR的变化 血脑屏障短暂破坏后的分布体积 通过非致死性高渗性颈动脉内输注。 提出了完全类似的23 Na MR研究。 这些将雇用 光谱成像技术，使直接分布体积 转换试剂（SR）的图像。 预计的主要差异包括 下面的. 房室Na+交换非常缓慢， 对23Na光谱图像没有影响。 电子收费 在体内SR阴离子的浓度大于CR阴离子的浓度。 因此，在本发明中， 看看前者的分布量是否 与后者相比，它们是有限的。 这项工作涉及物理学、物理化学和合成化学， 生物物理学和生理学，并在一些研究中产生了影响， 包括神经和心血管疾病在内的病理状况 紊乱