NONDIAMAGNETIC AGENTS IN IN VIVO 23NA AND 1H20 MR

非抗磁剂体内 23NA 和 1H20 MR

• 批准号: 2176445
• 负责人: CHARLES S. SPRINGER
• 金额: $ 71.01万
• 依托单位: ASSOCIATED UNIV-BROOKHAVEN NATL LAB
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-04-01 至 1997-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2176445
• 关键词: Rana; alternatives to animals in research; bioengineering /biomedical engineering; biomagnetism; biophysics; blood brain barrier; cations; computer program /software; contrast media; image processing; ion transport; ionophores; laboratory mouse; laboratory rat; magnetic resonance imaging; mathematical model; neuroanatomy; nuclear magnetic resonance spectroscopy; phantom model; pharmacokinetics; relaxation spectrometry; sodium; sodium channel; toad; water; yeasts

The work proposed here will continue to explore and demonstrate the range of applications of the effects of non-diamagnetic agents on the two strongest tissue NMR signals: those of 1H2O and 23Naaq. Specific projects in two general categories are proposed. It will be demonstrated that one can make 1H2O MR images of contrast reagent (CR) distribution volumes directly. This is a very important goal that has not yet been achieved in MRI. It is fundamental, and of utmost significance to more sophisticated applications of CRs. The new technique of combined relaxography and imaging (CRI), recently introduced by this laboratory, is key to the production of distribution volume images. It is explained in this proposal. Studies of cell suspension phantoms, mice, and rats are proposed to demonstrate this approach in vivo. The CRI technique is totally general and applicable to longitudinal, transverse, or rotating-frame NMR relaxation and can be used to study any CR, whether employing the hyperfine or the bulk magnetic susceptibility mechanisms (or both). Longitudinal relaxation and hyperfine CRs are emphasized in this proposal. The yeast cell suspension and rodent studies proposed will also allow the quantitative determination of the extent of compartmental H2O exchange and should permit and production of exchange maps in vivo. The rodent experiments will allow very quantitative measurements of changes in brain CR distribution volumes upon transient disruption of the blood-brain-barrier by non-lethal hyperosmolar intracarotid infusion. Completely analogous 23Na MR studies are proposed. These will employ spectroscopic imaging techniques to make direct distribution volume images of shift reagents (SRs). The main differences expected include the following. Compartmental Na+ exchange is extremely slow and will have no effect on the 23Na spectroscopic images. The electronic charges of the in vivo SR anions are greater than those of the CR anions. Thus, it will be interesting to see if the distribution volumes of the former are at all restricted when compared with those of the latter. This work involves aspects of physics, physical and synthetic chemistry, biophysics, and physiology and has ramifications in the study of a number of pathological conditions including neurological and cardiovascular disorders.

这里提出的工作将继续探索和展示范围 非抗磁性剂对两者的影响的应用 最强的组织 NMR 信号：1H2O 和 23Naaq 的信号。 具体的 提议的项目分为两大类。 将证明可以制作具有对比度的 1H2O MR 图像 试剂（CR）直接分配体积。 这是一个非常重要的 MRI 尚未实现的目标。 这是根本性的，并且 对于 CR 更复杂的应用至关重要。 新的 最近推出的联合松弛成像和成像 (CRI) 技术 通过这个实验室，是生产分布量的关键 图像。 该提案对此进行了解释。 细胞悬浮液的研究 建议使用模型、小鼠和大鼠来证明这种方法 体内。 CRI 技术是完全通用的，适用于 纵向、横向或旋转框架 NMR 弛豫，可以 用于研究任何 CR，无论是使用超精细还是批量 磁化率机制（或两者）。 纵向松弛 该提案强调了超精细 CR。 酵母细胞 拟议的悬浮和啮齿动物研究也将允许定量 确定隔室 H2O 交换的程度，并应 体内交换图的许可和制作。 啮齿动物实验 将允许对大脑 CR 的变化进行非常定量的测量 血脑屏障短暂破坏时的分布体积 通过非致死性高渗颈动脉内输注。 提出了完全类似的 23Na MR 研究。 这些将雇用 光谱成像技术制作直接分布体积 位移试剂 (SR) 的图像。 预期的主要差异包括 下列。 房室 Na+ 交换极其缓慢，并且会 对 23Na 光谱图像没有影响。 电子收费 体内SR阴离子的数量大于CR阴离子的数量。 因此， 看看前者的分布量是否会很有趣 与后者相比，完全受到限制。 这项工作涉及物理学、物理和合成化学等方面， 生物物理学和生理学，并在许多研究中产生影响 病理状况，包括神经系统和心血管系统 失调。