VIBRATIONAL STUDIES OF ENERGY TRANSDUCING PROTEINS

能量转换蛋白的振动研究

基本信息

  • 批准号:
    2180028
  • 负责人:
  • 金额:
    $ 11.12万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1988
  • 资助国家:
    美国
  • 起止时间:
    1988-07-01 至 1996-06-30
  • 项目状态:
    已结题

项目摘要

The specific aim of the proposed research is to characterize the structural and electronic properties of the active pigments in energy transducing proteins. The bacterial photosynthetic reaction center (RC) has been chosen for study because this class of proteins represents a prototypical model system for investigating the properties that facilitate fast electron transfer over distances on the order of the width of the biological membrane. In addition, the RC is the only integral membrane protein for which the macromolecular arrangement of the active pigments in the protein matrix is known. The principal investigative tool that will be used is resonance Raman (RR) spectroscopy. The first objective is to characterize the UV-vis-excitation (ca. 350-700 nm) RR spectra of the bacterochlorphyll (BChl) and bacteriopheophytin (BPh) pigments is RCs from Rb. sphaeroides, Rb. capsulatus and Rps. viridis wild-type. The goal of these studies is to examine the individual BChl and BPh molecules with as much selectivity as possible in order to assess the structural similarities and/or differences between the various pigments in RCs from a given species and between RCs from different species. The second objective is to characterize the UV- vis-excitation RR spectra of a number of genetically modified RCs from Rb. capsulatus. These studies will focus on systems in which genetic modifications have been made in the amino acid residues in the vicinity of the primary electron donor and acceptor and also the accessory BChls. These studies will be conducted in parallel with those on the wild-type systems in order to assess the structural consequences of the various site- specific mutations. The third objective is to probe the red-most, Qy absorptions (ca. 750-1000 nm) of the BChls and BPhs by using RR techniques. The red-most band of the primary donor (ca. 850-1000 nm) will be the focal point of the near-infrared (NIR) RR studies. The goal of these studies is to characterize the vibrational and electronic properties of the lowest- energy electronic states. The fourth objective is to obtain NIR-excitation RR spectra of RCs in which genetical modifications have been made in the vicinity of the photophysically active pigments. The goal of these studies is to determine how modifications in specific amino acids influence the vibrational and electronic properties of the electron-donating excited state of RCs.
拟议的研究的具体目的是表征结构 能量转换中活性颜料的电学性质 蛋白质。细菌光合作用反应中心(RC) 之所以选择进行研究是因为这类蛋白质代表了一种原型 用于研究促进快电子的性质的模型系统 按生物体宽度的数量级进行距离转移 薄膜。此外,RC是唯一一种完整的膜蛋白 蛋白质中活性色素的大分子排列 矩阵是已知的。将使用的主要调查工具是 共振拉曼(RR)光谱。第一个目标是描述 细菌叶绿素的UV-Vis激发(约350-700 nm)RR光谱 (BCHL)和细菌素(BPH)色素是RB的RCS。Shaeroids, RB。囊状细胞和RPS。绿色野生型。这些研究的目标是 检查单个BCHL和BPH分子,其选择性与 有可能评估结构上的相似性和/或差异 特定物种的RCS中的各种色素之间以及RCS之间 来自不同的物种。第二个目标是表征紫外线- 几种Rb转基因RCS的可见光激发RR谱 胶囊。这些研究将集中在基因 已对附近的氨基酸残基进行了修改 主要的电子供体和受体,以及辅助的BChls。 这些研究将与野生型的研究并行进行。 系统,以评估各种场地的结构后果- 特定的突变。第三个目标是探索最红的,Qy 用RR技术测定BChl和BPHS的吸收(约750-1000 nm)。 初级施主的最红波段(约850-1000 nm)将成为焦点。 近红外(NIR)RR研究的要点。这些研究的目标是 为了表征最低的原子的振动和电子性质- 能量电子态。第四个目标是获得近红外激发。 经遗传修饰的RCS的RR谱 光物理活性色素的附近。这些研究的目标是 是确定特定氨基酸的修饰如何影响 给电子激发态的振动和电学性质 RCS的状态。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

DAVID F. BOCIAN其他文献

DAVID F. BOCIAN的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('DAVID F. BOCIAN', 18)}}的其他基金

VIBRATIONAL STUDIES OF ENERGY TRANSDUCING PROTEINS
能量转换蛋白的振动研究
  • 批准号:
    3296956
  • 财政年份:
    1988
  • 资助金额:
    $ 11.12万
  • 项目类别:
VIBRATIONAL STUDIES OF ENERGY TRANSDUCING PROTEINS
能量转换蛋白的振动研究
  • 批准号:
    2180030
  • 财政年份:
    1988
  • 资助金额:
    $ 11.12万
  • 项目类别:
VIBRATIONAL STUDIES OF ENERGY TRANSDUCING PROTEINS
能量转换蛋白的振动研究
  • 批准号:
    6525600
  • 财政年份:
    1988
  • 资助金额:
    $ 11.12万
  • 项目类别:
VIBRATIONAL STUDIES OF ENERGY TRANSDUCING PROTEINS
能量转换蛋白的振动研究
  • 批准号:
    2180029
  • 财政年份:
    1988
  • 资助金额:
    $ 11.12万
  • 项目类别:
VIBRATIONAL STUDIES OF ENERGY TRANSDUCING PROTEINS
能量转换蛋白的振动研究
  • 批准号:
    6612528
  • 财政年份:
    1988
  • 资助金额:
    $ 11.12万
  • 项目类别:
VIBRATIONAL STUDIES OF ENERGY TRANSDUCING PROTEINS
能量转换蛋白的振动研究
  • 批准号:
    6192238
  • 财政年份:
    1988
  • 资助金额:
    $ 11.12万
  • 项目类别:
VIBRATIONAL STUDIES OF ENERGY TRANSDUCING PROTEINS
能量转换蛋白的振动研究
  • 批准号:
    3296958
  • 财政年份:
    1988
  • 资助金额:
    $ 11.12万
  • 项目类别:
VIBRATIONAL STUDIES OF ENERGY TRANSDUCING PROTEINS
能量转换蛋白的振动研究
  • 批准号:
    3296957
  • 财政年份:
    1988
  • 资助金额:
    $ 11.12万
  • 项目类别:
VIBRATIONAL STUDIES OF ENERGY TRANSDUCING PROTEINS
能量转换蛋白的振动研究
  • 批准号:
    3296955
  • 财政年份:
    1988
  • 资助金额:
    $ 11.12万
  • 项目类别:
VIBRATIONAL STUDIES OF ENERGY TRANSDUCING PROTEINS
能量转换蛋白的振动研究
  • 批准号:
    6385722
  • 财政年份:
    1988
  • 资助金额:
    $ 11.12万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了