GENETIC CONTROL OF MICROTUBULE DYNAMICS AND FUNCTION

本提案的广泛、长期目标是了解 调节酵母中微管动力学的分子机制 酿酒酵母和这个过程如何控制染色体 细胞分裂时的行为。 该提案将侧重于遗传 CDC20基因的相互作用和调控，微管的调节器 拆卸 该补助金的具体目标是：1）。 以识别 与Cdc2Op相互作用的蛋白质和与CDC 20的遗传相互作用。 2）。 建立新型的微管在体内和体外的检测方法 并将其应用于突变体。3）。 确定 调节CDC20表达的机制，以及它是否对 功能4）。 描述CDC 20检查点的特征并确定如何 CDC20的功能被整合到细胞周期中。 主要量的 将致力于分离和表征突变体， 克隆同源基因。 这些努力将奠定重要基础 用于未来的实验。 微管在结构上是普遍存在的， 真核细胞中的功能及其调节机制 动态行为，并将其功能整合到细胞周期中， 对理解有丝分裂至关重要， 进化上保守。

The broad, long term objectives of this proposal are to understand the molecular mechanisms that regulate microtubule dynamics in the yeast Saccharomyces cerevisiae and how this process controls chromosome behavior during cell division. This proposal will focus on genetic interactions and regulation of the CDC20 gene, a regulator of microtubule disassembly. The Specific Aims of this grant are: 1). To identify proteins that interact with Cdc2Op and genetic interactions with CDC20. 2). To establish novel in vivo and in vitro assays for microtubule dynamics in yeast and apply them to mutants. 3). To determine the mechanism(s) of regulated CDC20 expression and if it is essential for function. 4). To characterize the CDC20 checkpoint and determine how CDC20 function is integrated into the cell cycle. A major amount of the effort will be devoted to isolating and characterizing mutants and cloning the cognate genes. These efforts will lay important groundwork for future experiments. Microtubules are ubiquitous in structure and function in eucaryotic cells and the mechanisms that regulate their dynamic behavior and integrate their function into the cell cycle are of central importance to understanding mitosis and are likely to be evolutionarily conserved.