PROBES OF STRUCTURE & MECHANISM IN COPPER AMINE OXIDASES

从牛血浆中提取的铜胺氧化酶最近被发现 含有2，4，5-三羟基苯丙氨酸(TOPA)作为活性中心，氧化还原 辅因。这一发现开辟了许多新的研究途径。 预计授权期的具体目标包括： (1)建立TOPA作为铜中氧化还原辅因子的一般性 来自各种来源(细菌、酵母和哺乳动物)的胺氧化酶 组织)； (2)测定含有TOPA的多肽序列，以确定 协商一致序列的存在和性质； (3)鉴定编码TopA辅因子的DNA序列； (4)建立TOPA的生物发生途径； (5)阐明了TOPA模型的物理化学性质 化合物；以及 (6)研究TOPA的反应性和催化性能。 牛血浆胺氧化酶的活性部位。

The copper amine oxidase from bovine plasma has recently been found to contain 2,4,5-trihydroxyphenylalanine (topa) as the active site, redox cofactor. This discovery opens up many new avenues of research. Specific aims for the projected granting period include: (1) Establishing the generality of topa as a redox cofactor in copper amine oxidases from a variety of sources (bacteria, yeast and mammalian tissue); (2) Determining the sequences of topa containing peptides, to ascertain the presence and nature of a consensus sequence; (3) Identifying the DNA sequence encoding the topa cofactor; (4) Establishing the pathway of topa biogenesis; (5) Elucidating the physical and chemical properties of topa model compounds; and (6) Pursuing the reactivity and catalytic properties of topa at the active site of bovine plasma amine oxidase.