PROBES OF STRUCTURE & MECHANISM IN COPPER AMINE OXIDASES

结构探针

• 批准号: 3296146
• 负责人: JUDITH P KLINMAN
• 金额: $ 18.9万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-01 至 1996-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3296146
• 关键词: active sites; amine oxidase (flavin); animal tissue; bacteria; cofactor; copper; covalent bond; enzyme mechanism; enzyme structure; high performance liquid chromatography; hydroxyaminoacid; mass spectrometry; metalloenzyme; nuclear magnetic resonance spectroscopy; nucleic acid sequence; oxidation reduction reaction; phenylalanine analog; protein sequence; ultraviolet spectrometry; yeasts

The copper amine oxidase from bovine plasma has recently been found to contain 2,4,5-trihydroxyphenylalanine (topa) as the active site, redox cofactor. This discovery opens up many new avenues of research. Specific aims for the projected granting period include: (1) Establishing the generality of topa as a redox cofactor in copper amine oxidases from a variety of sources (bacteria, yeast and mammalian tissue); (2) Determining the sequences of topa containing peptides, to ascertain the presence and nature of a consensus sequence; (3) Identifying the DNA sequence encoding the topa cofactor; (4) Establishing the pathway of topa biogenesis; (5) Elucidating the physical and chemical properties of topa model compounds; and (6) Pursuing the reactivity and catalytic properties of topa at the active site of bovine plasma amine oxidase.

最近发现牛血浆中的铜胺氧化酶 含有2，4，5-三羟基苯丙氨酸（topa）作为活性位点， 辅因子 这一发现开辟了许多新的研究途径。 预计赠款期的具体目标包括： (1)建立topa作为铜中氧化还原辅因子的一般性 来自多种来源（细菌、酵母和哺乳动物）的胺氧化酶 组织）; (2)确定含有topa的肽的序列，以确定 共有序列的存在和性质; (3)鉴定编码topa辅因子的DNA序列; (4)建立topa生物合成途径; (5)TOPA模型的物理化学性质解析 化合物;以及 (6)研究了topa的反应性和催化性能， 牛血浆胺氧化酶的活性部位。