EFFECTORS OF ORGANIZATION OF TRANSMEMBRANE PROTEINS

跨膜蛋白组织的影响因子

• 批准号: 2179299
• 负责人: MARINO MARTINEZ-CARRION
• 金额: $ 15.11万
• 依托单位: UNIVERSITY OF MISSOURI KANSAS CITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-12-01 至 1995-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2179299
• 关键词: X ray crystallography; acetylcholine; aminoacid; aspartate transaminase; binding proteins; conformation; extracellular matrix proteins; fluorescent dye /probe; ligands; membrane channels; membrane lipids; membrane permeability; membrane proteins; mitochondrial membrane; monoclonal antibody; mutant; neurotoxins; nicotinic receptors; phospholipids; protein sequence; protein structure; proteolysis; vesicle /vacuole; western blottings

The conformation of constitutive membrane proteins is poorly understood and the effect of membrane lipids on these proteins, or on those that must traverse membranes in intracellular traffic, is also in need of clarification. In this project, two proteins, the acetylcholine receptor (AcChR) and a precursor to a mitochondrial matrix protein, aspartate aminotransferase (pmAAT), are used as models to gain greater understanding of the above problems. In the first case, particular attention is paid to the development of quantitative techniques to assign specific lysyl residues in the sequence of all the constitutive subunits to the cytoplasmic side of the membrane, which is a region largely unexplored in transmembranous proteins. Characterization of contact points between AcChR and specific neurotoxins and selected antibodies against synaptic (exterior) side will also be carried out, as well as the effect of such protein-protein interactions in the exposure of the (distant) cytoplasmic side lysyl residues. iN the second protein system, the availability of a precursor as an isolated, soluble, stable protein provides the first opportunity to observe precursor-membrane interactions under controlled conditions. Thus, in pmAAT, the role of the presequence consequences of such on precursor structure, will be assessed by antibodies and a variety of biophysical tools.

构成膜蛋白的构象是知之甚少和