PYRIDOXAL AS A PROBE MOLECULAR FUNCTION

拟议研究的目标是：提出新的研究方法 PLP作为其他蛋白质的特异性修饰剂的选择性应用 溶液或膜。开发确定溶液性质的方法 游离酶和酶-底物中活性部位的氨基酸残基 复合体。确定醛以外的结构基团的作用 和酶-辅酶关系中的氨基，甚至它们的 酶催化差异在酶功能上的合理含义 反应的类型。以确定构成活性氨基酸的特征 中心选定的磷酸吡哆醛依赖酶的例子。为了澄清 这些氨基酸(d点)在酶-辅酶-底物中的作用 与催化的关系和机理。要检测所产生的影响 通过相互作用的蛋白质在战略蛋白质区域的微环境 依赖于PLP的蛋白或结合的吡哆醛磷酸中的基团 (PLP)或磷酸吡哆胺(PMP)分子。利用对…的偏爱 PLP通过不需要PLP的蛋白质来加深我们对局部化的认识 配基与蛋白质相互作用引起的扰动 蛋白质间的异位相互作用。

The objectives of the proposed research are: To bring about new methodology for the selective use of PLP as a specific modifying agent of other proteins in solution or membranes. To develop methods to determine the solution properties of amino acid residues at active sites in both free enzymes and enzyme-substrate complexes. To identify the role of structural groups other than the aldehyde and amino groups in the coenzyme in enzyme-coenzyme relationships and even their plausible implication in enzymatic function for enzyme catalyzing different types of reactions. To characterize those amino acids constituting the active center of selected examples of phosphopyridoxal dependent enzymes. To clarify the role of those amino acids (point d) in enzyme-coenzyme-substrate relationships and the mechanism of catalysis. To detect the effects ellicited by interacting proteins on the microenvironment of strategic protein regions of the PLP-dependent protein or on groups within the bound pyridoxal phosphate (PLP) or pyridoxamine phosphate (PMP) molecules. To exploit the preference for PLP by non PLP-requiring proteins to further our knowledge of localized perturbations resulting from ligand interactions with the protein during heterotopic protein-protein interactions.