PYRIDOXAL AS A PROBE MOLECULAR FUNCTION

吡哆醛作为探针分子功能

• 批准号: 3294323
• 负责人: MARINO MARTINEZ-CARRION
• 金额: $ 6.31万
• 依托单位: UNIVERSITY OF MISSOURI KANSAS CITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-01 至 1988-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3294323
• 关键词: aspartate carbamoyltransferase; aspartate transaminase; cofactor; enzyme structure; enzyme substrate complex; glutamate decarboxylase; intermolecular interaction; membrane structure; molecular rearrangement; neurotoxins; nutrition related tag; pyridoxal phosphate; vitamin biosynthesis; vitamin metabolism

The objectives of the proposed research are: To bring about new methodology for the selective use of PLP as a specific modifying agent of other proteins in solution or membranes. To develop methods to determine the solution properties of amino acid residues at active sites in both free enzymes and enzyme-substrate complexes. To identify the role of structural groups other than the aldehyde and amino groups in the coenzyme in enzyme-coenzyme relationships and even their plausible implication in enzymatic function for enzyme catalyzing different types of reactions. To characterize those amino acids constituting the active center of selected examples of phosphopyridoxal dependent enzymes. To clarify the role of those amino acids (point d) in enzyme-coenzyme-substrate relationships and the mechanism of catalysis. To detect the effects ellicited by interacting proteins on the microenvironment of strategic protein regions of the PLP-dependent protein or on groups within the bound pyridoxal phosphate (PLP) or pyridoxamine phosphate (PMP) molecules. To exploit the preference for PLP by non PLP-requiring proteins to further our knowledge of localized perturbations resulting from ligand interactions with the protein during heterotopic protein-protein interactions.

拟议研究的目标是： 选择性使用PLP作为其他蛋白质的特异性修饰剂， 溶液或膜。 开发确定溶液性质的方法 游离酶和酶底物中活性位点的氨基酸残基 配合物 确定醛以外的结构基团的作用 在酶-辅酶关系中的辅酶和氨基， 在酶的功能中，酶催化不同的 反应的类型。 为了表征构成活性成分的氨基酸， 磷酸吡哆醛依赖酶的选定实例的中心。 澄清 这些氨基酸（点d）在酶-辅酶-底物中作用 关系和催化机制。 为了检测所引发的影响， 通过相互作用的蛋白质的战略蛋白质区域的微环境， PLP依赖性蛋白或结合磷酸吡哆醛内的基团 (PLP)或磷酸吡哆胺（PMP）分子。 利用对…的偏爱 PLP通过非PLP需要的蛋白质，以进一步我们的知识， 由于配体与蛋白质的相互作用而引起的扰动， 异位蛋白质间相互作用。