TRANSIENT CIRCULAR DICHROISM STUDIES OF PROTEIN MOTIONS
TRANSIENT CIRCULAR DICHROISM STUDIES OF PROTEIN MOTIONS
批准号:
2181147
负责人:
JOHN D SIMON
金额:
$10.39万
依托单位国家:
美国
项目类别:
财政年份:
1990
资助国家:
美国
项目状态:
已结题
起止时间:
1990-01-01 至 1997-06-30
关键词:
band 3 protein bioengineering /biomedical engineering biomedical equipment development carboxyhemoglobin chemical association chemical models circular dichroism conformation cytochrome c hemoglobin hemoprotein laser spectrometry myoglobin oxidation reduction reaction photochemistry photolysis protein folding protein structure function stop flow technique synthetic peptide time resolved data tryptophan ultraviolet radiation
中文摘要
时间分辨圆二色光谱(TRCD)是一种新的
我们在实验室开发的实验技术,
蛋白质构象动力学研究。 该提案提出
利用三氯化碳和四氯化碳进行的生物化学过程的持续研究和新研究,
互补的时间分辨激光光谱。
在赠款期间,与
TRCD技术及其光谱学应用
将研究生物分子中的构象运动。 是
我们的目标是开发收集瞬态紫外线的技术,
圆二色谱 我们的方法的实验细节是
概述。 建议的改善措施将可收集
在205 nm和250 nm之间的紫外圆二色性光谱,在任何
所需的时间延迟在30皮秒和10微秒之间
光激发 光谱的这一区域监测着
蛋白质骨架的吸收。 紫外CD光谱
提供了蛋白质中存在的二级结构的量度,
溶液 对血红素蛋白质的弛豫的继续研究
将进行配体光解离。 在此期间的主要努力
下一个资助期将侧重于使用紫外CD技术,沿着
各种激光光谱探针来检查(a)次级
蛋白质折叠和解折叠中的结构进化和(B)
小多肽中光诱导的螺旋-卷曲转变。 蛋白
折叠是生物化学中的一个重要问题。 的应用
时间分辨UV CD光谱对这个问题提供了产生新的
和令人兴奋的洞察力的时间尺度与形成
蛋白质二级结构 还将进行研究,
开发新的光化学方法来触发蛋白质解折叠
和折叠过程。
英文摘要
Time-resolved circular dichroism (TRCD) spectroscopy is a new
experimental technique that we have developed in our laboratory for the
study of protein conformational dynamics. This proposal presents
continued and new studies of biochemical processes using TRCD and
complementary time-resolved laser spectroscopies.
During the grant period, both technical developments associated with the
TRCD technique and applications of this spectroscopy to the study of
conformational motions in biological molecules will be pursued. It is
our goal to develop the technology for collecting transient ultraviolet
circular dichroism spectra. The experimental details of our approach are
outlined. The proposed improvements will enable collection of
ultraviolet circular dichroism spectra between 205 nm and 250 nm, at any
desired time delay between 30 picoseconds and 10 microseconds following
photoexcitation. This region of the optical spectrum monitors the
absorption of the protein backbone. CD spectra in the ultraviolet
provide a measure of the secondary structures present in proteins in
solution. Continued studies on the relaxation of heme proteins following
ligand photodissociation will be conducted. The major effort during this
next grant period will focus on using the UV CD technique along with a
variety of laser spectroscopic probes to examine the (a) secondary
structure evolution in the folding and unfolding of proteins and (b)
photo-induced helix-coil transitions in small polypeptides. Protein
folding is an important problem in biochemistry. The application of
time resolved UV CD spectroscopy to this problem offers to produce new
and exciting insights into the time scales associated with the formation
of protein secondary structure. Research will also be conducted aimed
at developing new photochemical methods for triggering protein unfolding
and folding processes.
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会议论文
PHOTODYNAMICS OF ULTRAVIOLET EPIDERMAL CHROMOPHORES
-
批准号:6386803
-
项目类别:
-
资助金额:$16.93万
-
财政年份:1998
-
负责人:JOHN D SIMON
-
依托单位:
PHOTODYNAMICS OF ULTRAVIOLET EPIDERMAL CHROMAPHORES
-
批准号:2693277
-
项目类别:
-
资助金额:$20.02万
-
财政年份:1998
-
负责人:JOHN D SIMON
-
依托单位:
PHOTODYNAMICS OF ULTRAVIOLET EPIDERMAL CHROMOPHORES
-
批准号:6605400
-
项目类别:
-
资助金额:$5.64万
-
财政年份:1998
-
负责人:JOHN D SIMON
-
依托单位:
PHOTODYNAMICS OF ULTRAVIOLET EPIDERMAL CHROMAPHORES
-
批准号:6019396
-
项目类别:
-
资助金额:$16.07万
-
财政年份:1998
-
负责人:JOHN D SIMON
-
依托单位:
PHOTODYNAMICS OF ULTRAVIOLET EPIDERMAL CHROMOPHORES
-
批准号:6181095
-
项目类别:
-
资助金额:$16.5万
-
财政年份:1998
-
负责人:JOHN D SIMON
-
依托单位:
TRANSIENT CIRCULAR DICHROISM STUDIES OF PROTEIN MOTIONS
-
批准号:3300444
-
项目类别:
-
资助金额:$11.63万
-
财政年份:1990
-
负责人:JOHN D SIMON
-
依托单位:
PICOSECOND CIRCULAR DICHROISM STUDIES OF PROTEIN MOTION
-
批准号:3300445
-
项目类别:
-
资助金额:$8.09万
-
财政年份:1990
-
负责人:JOHN D SIMON
-
依托单位:
PICOSECOND CIRCULAR DICHROISM STUDIES OF PROTEIN MOTION
-
批准号:3300443
-
项目类别:
-
资助金额:$7.15万
-
财政年份:1990
-
负责人:JOHN D SIMON
-
依托单位:
PICOSECOND CIRCULAR DICHROISM STUDIES OF PROTEIN MOTION
-
批准号:3300446
-
项目类别:
-
资助金额:$8.36万
-
财政年份:1990
-
负责人:JOHN D SIMON
-
依托单位:
TRANSIENT CIRCULAR DICHROISM STUDIES OF PROTEIN MOTIONS
-
批准号:2181148
-
项目类别:
-
资助金额:$10.81万
-
财政年份:1990
-
负责人:JOHN D SIMON
-
依托单位:
TRANSIENT CIRCULAR DICHROISM STUDIES OF PROTEIN MOTIONS
-
批准号:2181149
-
项目类别:
-
资助金额:$11.29万
-
财政年份:1990
-
负责人:JOHN D SIMON
-
依托单位: