PICOSECOND CIRCULAR DICHROISM STUDIES OF PROTEIN MOTION

蛋白质运动的皮秒圆二色性研究

• 批准号: 3300445
• 负责人: JOHN D SIMON
• 金额: $ 8.09万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-01-01 至 1992-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3300445
• 关键词: chemical models; conformation; electron transport; hemoglobin; isomer; model; myoglobin; protein structure; rhodopsin; spectrometry

Time resolved circular dichroism spectroscopy is a technique that we have developed in our laboratory for the study of protein conformational dynamics. The capabilities of our experimental approach allows one to measure conformational motions of proteins that occur on the picosecond or slower time scale. In this proposal, we discuss, in detail, the experimental approach for measuring time resolved circular dichroism spectra on the picosecond time scale and applications of our technique to the study of protein conformation changes caused by the photoinduced debinding of substrates bound to myoglobin, hemoglobin and model systems. In addition, we discuss future applications of our new experimental technique to determine (1) protein motions induced by the isomerization of retinal in the visual protein rhodopsin, and (2) changes in protein structure caused by electron transfer chemistry in hybrid hemoglobins.

时间分辨圆二色光谱是一种技术， 本实验室开发的用于研究蛋白质构象的 动力学 我们的实验方法的能力允许一个 测量蛋白质的构象运动，发生在皮秒或 慢的时间尺度。 在这个建议中，我们详细讨论了实验方法， 皮秒时间分辨圆二色光谱的测量 我们的技术在蛋白质研究中的规模和应用 底物光诱导脱粘引起的构象变化 与肌红蛋白、血红蛋白和模型系统结合。 此外，我们讨论 我们的新实验技术的未来应用，以确定（1） 视神经中由视网膜异构化引起的蛋白质运动 蛋白质视紫红质，和（2）由电子引起的蛋白质结构的变化 杂交血红蛋白中的转移化学