FOLDING AND DYNAMICS OF A BI-DOMAIN ENZYME
FOLDING AND DYNAMICS OF A BI-DOMAIN ENZYME
批准号:
2180828
负责人:
MARIA T MAS
金额:
$22.94万
依托单位国家:
美国
项目类别:
财政年份:
1988
资助国家:
美国
项目状态:
已结题
起止时间:
1988-12-01 至 1998-06-30
关键词:
chemical kinetics circular dichroism conformation enzyme structure fluorescence spectrometry fluorescent dye /probe laser spectrometry molecular dynamics mutant phosphoglycerate kinase protein engineering protein folding protein purification site directed mutagenesis stop flow technique time resolved data
中文摘要
本项目的目的是表征折叠机制的两个-
结构域酶,3-磷酸甘油酸激酶(PGK)。 实验方法
包括基因工程、稳态和时间的结合-
分辨荧光和圆二色性技术。 时间分辨
将进行荧光能量转移测量,以便
确定关键的域内和域间距离对之间的
用外源荧光标记的基因工程半胱氨酸
probes. 基因工程改造的基因组将被用作
在展开过程中监测局部和全局变化的探针,
重折叠过渡 上述方法预计将提供一个
天然、中间和未折叠状态的详细表征,
以及形成的时间顺序(i)个别中学
结构元素,(ii)单个域和(iii)域-域
接口. 这项研究的长期目标是解开信息
包含在指导这个折叠的氨基酸序列中,
酵素
英文摘要
The aim of this project is to characterize the folding mechanism of a two-
domain enzyme, 3-phosphoglycerate kinase (PGK). The experimental approach
involves a combination of genetic engineering, steady-state and time-
resolved fluorescence, and circular dichroism techniques. Time-resolved
fluorescence energy transfer measurements will be carried out in order to
determine the key intra- and inter-domain distances between pairs of
genetically-engineered cysteines, labeled with extrinsic fluorescent
probes. Genetically-engineered tryptophans will be used as intrinsic
probes to monitor local and global changes during the unfolding and
refolding transitions. The above approach is expected to provide a
detailed characterization of the native, intermediate and unfolded states,
as well as the time-sequence of the formation of (i) individual secondary
structure elements, (ii) individual domains and (iii) the domain-domain
interface. The long range goal of this study is to unravel information
contained in the amino acid sequence that direct the folding of this
enzyme.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
FOLDING AND DYNAMICS OF A BI-DOMAIN ENZYME
-
批准号:2180830
-
项目类别:
-
资助金额:$27.2万
-
财政年份:1988
-
负责人:MARIA T MAS
-
依托单位:
FOLDING AND DYNAMICS OF A BI-DOMAIN ENZYME
-
批准号:3299517
-
项目类别:
-
资助金额:$18.01万
-
财政年份:1988
-
负责人:MARIA T MAS
-
依托单位:
FOLDING AND DYNAMICS OF A BI-DOMAIN ENZYME
-
批准号:2444698
-
项目类别:
-
资助金额:$28.28万
-
财政年份:1988
-
负责人:MARIA T MAS
-
依托单位:
FOLDING AND DYNAMICS OF A BI-DOMAIN ENZYME
-
批准号:2180829
-
项目类别:
-
资助金额:$26.15万
-
财政年份:1988
-
负责人:MARIA T MAS
-
依托单位:
FOLDING AND DYNAMICS OF A BI-DOMAIN ENZYME
-
批准号:3299519
-
项目类别:
-
资助金额:$17.42万
-
财政年份:1988
-
负责人:MARIA T MAS
-
依托单位:
FOLDING AND DYNAMICS OF A BI-DOMAIN ENZYME
-
批准号:3299518
-
项目类别:
-
资助金额:$16.75万
-
财政年份:1988
-
负责人:MARIA T MAS
-
依托单位:
MECHANISM OF DOMAIN MOVEMENT IN PHOSPHOGLYCERATE KINASE
-
批准号:3293303
-
项目类别:
-
资助金额:$17.44万
-
财政年份:1986
-
负责人:MARIA T MAS
-
依托单位:
MECHANISM OF DOMAIN MOVEMENT IN PHOSPHOGLYCERATE KINASE
-
批准号:3293306
-
项目类别:
-
资助金额:$15.44万
-
财政年份:1986
-
负责人:MARIA T MAS
-
依托单位:
MECHANISM OF DOMAIN MOVEMENT IN PHOSPHOGLYCERATE KINASE
-
批准号:2178925
-
项目类别:
-
资助金额:$21.9万
-
财政年份:1986
-
负责人:MARIA T MAS
-
依托单位:
MECHANISM OF DOMAIN MOVEMENT IN PHOSPHOGLYCERATE KINASE
-
批准号:3293307
-
项目类别:
-
资助金额:$15.52万
-
财政年份:1986
-
负责人:MARIA T MAS
-
依托单位:
MECHANISM OF DOMAIN MOVEMENT IN PHOSPHOGLYCERATE KINASE
-
批准号:3293309
-
项目类别:
-
资助金额:$19.9万
-
财政年份:1986
-
负责人:MARIA T MAS
-
依托单位:
MECHANISM OF DOMAIN MOVEMENT IN PHOSPHOGLYCERATE KINASE
-
批准号:3293305
-
项目类别:
-
资助金额:$14.99万
-
财政年份:1986
-
负责人:MARIA T MAS
-
依托单位:
MECHANISM OF DOMAIN MOVEMENT IN PHOSPHOGLYCERATE KINASE
-
批准号:3293310
-
项目类别:
-
资助金额:$20.62万
-
财政年份:1986
-
负责人:MARIA T MAS
-
依托单位:
MECHANISM OF DOMAIN MOVEMENT IN PHOSPHOGLYCERATE KINASE
-
批准号:3293308
-
项目类别:
-
资助金额:$19.07万
-
财政年份:1986
-
负责人:MARIA T MAS
-
依托单位:
海外基金