课题基金 / 基金详情

CARBOHYDRATE DETERMINANTS IN TRANSFERRIN RECEPTOR ACTION

CARBOHYDRATE DETERMINANTS IN TRANSFERRIN RECEPTOR ACTION
转铁蛋白受体作用中的碳水化合物决定因素
批准号:
3302053
负责人:
JOHN J LUCAS
金额:
$24.44万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1990
资助国家:
美国
项目状态:
已结题
起止时间:
1990-07-01 至 1995-06-30

项目摘要

项目成果

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中文摘要
翻译
哺乳动物细胞对铁的吸收涉及到铁的结合
英文摘要
The uptake of iron into mammalian cells involves the binding of transferrin, the serum iron transport protein, to the tranferin receptor, followed by the internalization of the receptor-transferrin complex. Iron accumulation is essential for cell division. Rapidly proliferating cells have higher iron requirements than non-dividing cells and the receptor has been identified as a proliferation specific marker. The transferrin receptor contains three asparagine-linked oligosaccharides, which are a mixture of "high mannose" and "complex" structures and at least one O-linked (serine/threonine) oligosaccharide. Prior studies using tunicamycin-treated human cells have demonstrated that the unglycosylated from of the transferrin receptor is not functional. Thus, the goals of this proposal are to test the hypothesis that glycosylation is vital for the correct folding and transport of the transferrin receptor to the cell surface and in the binding or transferrin. Site-directed mutagenesis will be used to test the contribution of each of the asparagine-linked glycosylation sites to the folding and transport of the transferrin receptor. Combinations of single, double and triple mutations will be prepared and tested mouse cell lines. Evidence suggests that processing of oligosaccharides to complex forms enhances receptor function and the role of this processing will be examined in glycosylation deficient Chinese Hamster Ovary cell mutants. Such mutants will also be sued to investigate the role of O-linked glycosylation in receptor function. Transferrin receptor oligosaccharides have not been directly characterized and they will be analyzed with respect to structure (high mannose/complex) present on each of the asparagine-linked sites in the native transferrin receptor as well as the receptor expressed in murine and CHO cells following transfection. These analyses will be performed via carbohydrate and protein chemical techniques, including - reverse phase HPLC, peptides sequencing and analysis of oligosaccharides on lectin affinity and molecular sieve columns.
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SMALL INSTRUMENTATION GRANT
  • 批准号:
    3525656
  • 项目类别:
  • 资助金额:
    $4.46万
  • 财政年份:
    1991
  • 负责人:
    JOHN J LUCAS
  • 依托单位:
CARBOHYDRATE DETERMINANTS IN TRANSFERRIN RECEPTOR ACTION
  • 批准号:
    3302051
  • 项目类别:
  • 资助金额:
    $20.52万
  • 财政年份:
    1990
  • 负责人:
    JOHN J LUCAS
  • 依托单位:
CARBOHYDRATE DETERMINANTS IN TRANSFERRIN RECEPTOR ACTION
  • 批准号:
    3302052
  • 项目类别:
  • 资助金额:
    $22.33万
  • 财政年份:
    1990
  • 负责人:
    JOHN J LUCAS
  • 依托单位:
CARBOHYDRATE DETERMINANTS IN TRANSFERRIN RECEPTOR ACTION
  • 批准号:
    3302050
  • 项目类别:
  • 资助金额:
    $20.7万
  • 财政年份:
    1990
  • 负责人:
    JOHN J LUCAS
  • 依托单位:
海外基金