LIPIDS OF LEISHMANIAS AND TRYPANOSOMES

利什曼原虫和锥虫的脂质

• 批准号: 3124263
• 负责人: JOHN J LUCAS
• 金额: $ 12.87万
• 依托单位: UPSTATE MEDICAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-04-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3124263
• 关键词: Leishmania; antiprotozoal agents; azoles; cholesterol; communicable disease control; cytotoxicity; enzyme mechanism; gas chromatography; high performance liquid chromatography; host organism interaction; life cycle; lipid metabolism; macrophage; mass spectrometry; membrane lipids; microorganism culture; monoclonal antibody; parasitic disease chemotherapy; pharmacology; sterols; thin layer chromatography; trypanosomiasis

The long term objective of this project is to identify and to study novel chemotherapeutic approaches to the control of leishmanial and American trypanosomal infections of substantial medical importance, by exploiting knowledge that aspects of the lipid biochemistry of the responsible trypanosomatid flagellates are characteristic of fungi and other plants and not of vertebrates. The immediate specific aims to realize this objective are to investigate the: a) sterol, phospholipid and glycosphingolipid biochemistry of the life-cycle stages of representative leishmania species and Trypanosoma cruzi strains, b) perturbations of that biochemistry, and impairment of growth, cause by drugs targeted to specific enzymatic reactions, and c) mechanisms of action of the most effective of those drugs. Life-cycle stages are to be cultured within and without tissue cells exposed to drugs and radio- and stable isotopic tracers, and the consequences to growth and to lipid metabolism assessed by population enumeration, and by lipid analyses employing chromatographic (CC, TLC, HPLC, GLC), and spectrometric (GC/MS, 1H & 13C NMR, UV/VIS, liquid scintillation) methodologies. By these means, drugs active on enzymatic reactions unique to leishmanias and trypanosomes, or with properties different from those of analogous reactions in man, are to be identified, and their selective toxicities for the parasites and for host tissue cells established.

这个项目的长期目标是识别和研究

项目成果

Temperature-dependent fatty acyl group changes in phospholipids of 37 C-adapted Leishmania donovani promastigotes.

37 C 适应杜氏利什曼原虫前鞭毛体磷脂中温度依赖性脂肪酰基变化。

• 发表时间: 1982
• 期刊: The Journal of parasitology
• 作者: Beach,DH;HolzJr,GG;Semprevivo,LH;Honigberg,BM
• 通讯作者: Honigberg,BM