GENETIC ANALYSIS OF CHEMOSENSATION IN C ELEGANS
线虫化学感觉的遗传分析
基本信息
- 批准号:2186213
- 负责人:
- 金额:$ 9.46万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1993
- 资助国家:美国
- 起止时间:1993-08-01 至 1996-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The goal of the proposed research is to understand the cellular, genetic,
and molecular mechanisms of chemosensory response to the dauer-inducing
pheromone of Caenorhabditis elegans. The dauer larva is a developmentally
distinct alternative third-larval stage (L3) that is specialized for long-
term survival under harsh conditions. The choice between the normal L3 and
the dauer larva is controlled primarily by chemosensory assessment of the
environmental concentration of a secreted dauer-inducing pheromone. More
than 25 genes have been identified that control the process of dauer
formation and they have been placed into a complex epistasis pathway. Some
of these genes are implicated in the chemosensory process per se, while
others probably act downstream of chemosensation to activate the dauer-
larval developmental program. This proposal concentrates on the genes
implicated in the function and development of the dauer pheromone
chemosensory cells. One aim is to rigorously test the role of sensory
neurons that have been implicated in controlling pheromone response.
Pheromone responsiveness will be measured in animals in which individual
identified neurons have been eliminated with a laser microbeam. Another
aim is to complete a thorough search for mutations in genes important for
the function and development of these sensory cells. Mutations in genes
already implicated in pheromone chemosensation will be used to identify
other such genes using a variety of classical genetic approaches. One
gene, daf-11, already implicated specifically in the process of pheromone
chemosensation, will be cloned by transposon tagging. A variety of
molecular biological and genetic approaches will be taken to defining the
function of daf-11, including DNA sequence analysis, gene product
localization, and genetic mosaic analysis. Another gene, daf-19, is
implicated in the development of a large set of sensory neurons, including
those controlling dauer formation. daf-19 will be cloned using detailed
genetic and physical mapping and DNA transformation. The cloned gene will
be used to test the involvement of daf-19 in the development of the
sensory cells by DNA sequence analysis and expression studies.
Sensory modulation of dauer formation in C. elegans provides an excellent
model for the investigation of sensory and environmental influences on
development. The opportunity to both apply extensive genetic analysis to
such a problem and to define the sensory pathway at the single neuron
level is unique. In addition, response to dauer pheromone is a model for
the general process of chemosensation, which is currently poorly
understood.
这项研究的目标是了解细胞、遗传、
和分子机制的化学感应反应的道尔诱导
秀丽隐杆线虫信息素。Dauer幼虫是一种发育中的
不同的交替第三幼虫阶段(L3),专门用于长期-
在恶劣的条件下生存。正常L3和
Dauer幼虫主要通过化学感觉评估来控制,
环境浓度的一种分泌的诱导信息素。更
已经鉴定出25个以上的基因控制着dauer过程,
形成,它们被置于一个复杂的上位途径。一些
这些基因中的一个与化学感受过程本身有关,
其他的则可能在化学感觉的下游起作用以激活dauer-
幼虫发育程序这项建议集中在基因上,
与道尔信息素的功能和发育有关
化学感受细胞一个目的是严格测试感官的作用,
这些神经元与控制信息素反应有关。
信息素反应性将在动物中测量,其中个体
已识别的神经元已被激光微束消除。另一
目的是完成对重要基因突变的彻底搜索,
这些感觉细胞的功能和发育。基因突变
已经涉及信息素化学感觉将被用来识别
其他这样的基因使用各种经典的遗传方法。一
daf-11基因已经与信息素的产生过程有着密切的关系
chemosensation,将通过转座子标签克隆。各种
分子生物学和遗传学方法将被用来定义
daf-11的功能,包括DNA序列分析、基因产物
定位和遗传嵌合体分析。 另一个基因daf-19,
参与了大量感觉神经元的发育,包括
控制着Dauer的形成。daf-19将使用详细的
遗传和物理绘图以及DNA转化。克隆的基因将
用于测试daf-19参与开发
通过DNA序列分析和表达研究感觉细胞。
感觉调制的dauer形成的C。elegans提供了一个很好的
感官和环境影响调查模型
发展将广泛的基因分析应用于
并在单个神经元上定义感觉通路
水平是独一无二的。此外,对dauer信息素的反应是一个模型,
化学感觉的一般过程,目前很差,
明白
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('JAMES H THOMAS', 18)}}的其他基金
TISSUE DOPPLER ASSESSMENT OF RIGHT VENTRICULAR PERFORMANCE IN ACUTE HEART FAI
急性心衰患者右心室性能的组织多普勒评估
- 批准号:
7608224 - 财政年份:2007
- 资助金额:
$ 9.46万 - 项目类别:
NEUROLOGICAL TARGETS OF ANTIDEPRESSANTS IN C ELEGANS
线虫抗抑郁药的神经学靶标
- 批准号:
2697306 - 财政年份:1998
- 资助金额:
$ 9.46万 - 项目类别:
IDENTIFICATION OF NEURAL DEGENERATION MUTANTS OF C. ELEGANS
线虫神经退行性突变体的鉴定
- 批准号:
6098458 - 财政年份:1998
- 资助金额:
$ 9.46万 - 项目类别:
NEUROLOGICAL TARGETS OF ANTIDEPRESSANTS IN C ELEGANS
线虫抗抑郁药的神经学靶标
- 批准号:
6186613 - 财政年份:1998
- 资助金额:
$ 9.46万 - 项目类别:
NEUROLOGICAL TARGETS OF ANTIDEPRESSANTS IN C ELEGANS
线虫抗抑郁药的神经学靶标
- 批准号:
2891131 - 财政年份:1998
- 资助金额:
$ 9.46万 - 项目类别:
IDENTIFICATION OF NEURAL DEGENERATION MUTANTS OF C. ELEGANS
线虫神经退行性突变体的鉴定
- 批准号:
6234424 - 财政年份:1997
- 资助金额:
$ 9.46万 - 项目类别:
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