BIOCHEMISTRY AND GENETICS OF CALCIUM REGULATION IN YEAST

已知Ca2+具有几种重要的细胞内调节作用 在所有真核生物中。 一些细胞过程和功能 受Ca2+控制的细胞分化、细胞运动 内吞作用，形态发生，有丝分裂，分泌，细胞分裂，交配， 细胞和核聚变。 然而，Ca 2+在大多数细胞中的精确功能 这些进程尚未建立，情况是 因为所有这些Ca 2+介导的过程都发生在 彼此的结合。 模式真核生物S.酿酒酵母是 可能是最好的实验系统来剖析这些难以捉摸的 而是对Ca2+的普遍的细胞反应。 然而，要了解 要完全了解Ca 2+作为第二信使的作用，我们必须首先 阐明调节胞浆Ca 2+的机制 程度. 这将需要对机制的理解， Ca~（2+）跨膜运动和Ca~（2+）摄取的调节 并通过各种酵母细胞器释放。 该研究项目的具体目标是分离和 表征细胞质Ca 2+水平调节缺陷的突变体， 并利用这些突变体来识别基因（及其编码的蛋白质） 其负责维持正常的胞质Ca 2+水平。

Ca 2+ is known to have several important intracellular regulatory roles in all eucaryotes. Some of the cellular processes and functions controlled by Ca 2+ include cellular differentiation, cell movement, endocytosis, morphogenesis, mitosis, secretion, cell division, mating, cell and nuclear fusion. However, the precise function of Ca 2+ in most of these processes has not been established, and the situation is further complicated because all these Ca 2+ mediated processes occur in conjunction with one another. The model eucaryote, S. cerevisiae is probably the best experimental system in which to dissect these elusive but ubiquitous cellular responses to Ca 2+. However, to understand completely the role of Ca 2+ as a secondary messenger we must first elucidate the mechanisms responsible for regulating cytosolic Ca 2+ levels. This will require an understanding of the mechanism, and regulation of Ca 2+ movement across the plasma membrane and Ca 2+ uptake and release by the various yeast organelles. The specific goal of the research project is to isolate and characterize mutants defective in regulation of cytosolic Ca 2+ levels, and to use these mutants to identify genes (and their encoded proteins) which are responsible for maintaining normal cytosolic Ca 2+ levels.