BIOCHEMISTRY AND GENETICS OF CALCIUM REGULATION IN YEAST

已知钙离子2在细胞内有几个重要的调节作用。 在所有的真核生物中。一些细胞过程和功能 由钙控制的包括细胞分化、细胞运动、 内吞作用，形态发生，有丝分裂，分泌，细胞分裂，交配， 细胞和核融合。然而，在大多数情况下，钙的确切作用 这些进程中的一个尚未确定，目前的情况是 更复杂的是因为所有这些钙介导的过程都发生在 彼此联合起来。模式真核生物酿酒酵母是 可能是剖析这些难以捉摸的东西的最佳实验系统 但无处不在的细胞对钙的反应。然而，要理解 完全发挥钙作为第二信使的作用，我们必须首先 阐明细胞内钙离子的调节机制 级别。这需要对机制有一个了解，并且 钙跨膜转运和钙吸收的调节 并由各种酵母细胞器释放。 该研究项目的具体目标是隔离和 鉴定胞浆钙水平调节缺陷的突变体， 并使用这些突变体来识别基因(及其编码的蛋白质) 它们负责维持正常的胞浆钙水平。

Ca 2+ is known to have several important intracellular regulatory roles in all eucaryotes. Some of the cellular processes and functions controlled by Ca 2+ include cellular differentiation, cell movement, endocytosis, morphogenesis, mitosis, secretion, cell division, mating, cell and nuclear fusion. However, the precise function of Ca 2+ in most of these processes has not been established, and the situation is further complicated because all these Ca 2+ mediated processes occur in conjunction with one another. The model eucaryote, S. cerevisiae is probably the best experimental system in which to dissect these elusive but ubiquitous cellular responses to Ca 2+. However, to understand completely the role of Ca 2+ as a secondary messenger we must first elucidate the mechanisms responsible for regulating cytosolic Ca 2+ levels. This will require an understanding of the mechanism, and regulation of Ca 2+ movement across the plasma membrane and Ca 2+ uptake and release by the various yeast organelles. The specific goal of the research project is to isolate and characterize mutants defective in regulation of cytosolic Ca 2+ levels, and to use these mutants to identify genes (and their encoded proteins) which are responsible for maintaining normal cytosolic Ca 2+ levels.