BIOGENESIS OF THE YEAST MITOCHONDRIAL ATPASE COMPLEX

线粒体、叶绿体和细菌的H-ATPase合成ATP 在呼吸过程中，为细胞提供主要能源。一个 生物能量学领域中的一个重要问题涉及到 这种极其重要的酶。内部以及它们之间的相互作用 构成酶复合体的F1和F0组分起着密切的作用 在能量传递反应中的作用。关于ATPase的研究进展 生物发生学解决了功能重要性如何的根本问题 启动子单元联系。这个项目的长期目标是 鉴定并确定核基因产物的功能 酵母线粒体F1-F0复合体的生物合成 酿酒。基本目标是提供有关结构的信息 线粒体ATPase，并确定其结构如何影响 酶的功能。该项目还旨在回答 关于如何组装大分子蛋白质复合体的更具全球性的问题 从它们的组成亚基中分离出来。 目前的重点是专门组装的F1组件的 酵素。之前对F1组装中有缺陷的酵母突变株的研究 揭示了一类新的F1组装所需的蛋白质 齐聚物。一个直接的目标是提纯这些蛋白质，并将它们用于 将确定他们在F1组装中的角色的研究。基因外回复变种 已经从具有组装因子突变的酵母中分离出来 基因。抑制物将被克隆以识别相互作用的蛋白质 直接与F1的装配系数。 也有在F1组装中有缺陷的菌株，原因是 阿尔法和贝塔结构亚单位基因突变。这些变种人 将被用来获得基因外回复突变体。收购这些产品 返回者之所以重要，有两个原因。首先，这将确定 哪些α和β亚基突变体在以下区域受影响 直接与其他蛋白质相互作用。第二，识别 抑制亚基进入将揭示所需的蛋白质 将α和β亚基组装成F1寡聚体。

The H+ -ATPase of mitochondria, choloroplasts, and bacteria synthesizes ATP during respiration, providing the principal energy source for the cell. An important question in the field of Bioenergetics concerns the mechanism of this vitally important enzyme. The interactions within, and between, the F1 and F0 components that constitute the enzyme complex play an intimate role in the energy transduction reactions. The studies described on ATPase biogenesis address the fundamental question of how functionally important subunit contacts are initiated. The long-term goal of this project is to identify, and to define the function of, nuclear gene products required for biosynthesis of the mitochondrial F1-F0 complex in the yeast Saccharomyces cerevisiae. The basic objective is to provide information on the structure of the mitochondrial ATPase and to determine how the structure influences the function of the enzyme. The project is also aimed at answering the more global question of how macromolecular protein complexes are assembled from their component subunits. The present focus is specifically on assembly of the F1 component of the enzyme. Previous work with yeast mutants defective in F1 assembly has revealed a new class of proteins which are required for assembly of the F1 oligomer. An immediate goal is to purify these proteins and to use them in studies that will define their roles in F1 assembly. Extragenic revertants have already been isolated from yeast with mutations in the assembly factor genes. The suppressors will be cloned to identify proteins that interact directly with the F1 assembly factors. Strains are also available which are defective in F1 assembly due to mutations in the alpha and beta structural subunit genes. These mutants will be used to obtain extragenic revertants. The acquisition of these revertants will be important for two reasons. First, this will identify which of the alpha and beta subunit mutants are affected in domains that interact directly with other proteins. Second, identification of the suppressors subunits into will disclose proteins that are required for assembly of the alpha and beta subunits into the F1 oligomer.