BIOGENESIS OF THE YEAST MITOCHONDRIAL ATPASE COMPLEX

线粒体、叶绿体和细菌的H+ -ATP酶合成ATP 在呼吸过程中，为细胞提供主要的能量来源。 一个 生物能量学领域的一个重要问题是关于 这种极其重要的酶 内部和之间的相互作用， 构成酶复合物的F1和F0组分起着密切的作用。 在能量转换反应中的作用。 关于ATP酶的研究 生物起源论解决了一个基本问题， 启动子单元接触。 该项目的长期目标是 确定，并定义，所需的核基因产物的功能， 酵母中线粒体F1-F0复合物的生物合成 酿酒。 基本目标是提供有关结构的信息 的线粒体ATP酶，并确定结构如何影响 酶的功能。 该项目还旨在回答 更全球性的问题是大分子蛋白质复合物是如何组装的 从它们的组成子单元。 目前的重点是具体的F1组件的组装， 酵素 以前的工作与酵母突变体缺陷的F1装配， 揭示了一类新的蛋白质，这是需要组装的F1 低聚物 一个直接的目标是纯化这些蛋白质，并将它们用于 这些研究将确定它们在F1装配中的作用。 基因外回复突变体 已经从装配因子发生突变的酵母中分离出来 基因. 将克隆抑制基因以鉴定相互作用的蛋白质 与F1装配系数直接相关。 也可获得F1组装中存在缺陷的菌株，原因是 α和β结构亚基基因的突变。 这些突变体 将用于获得基因外回复突变体。 收购这些 回复突变体的重要性有两个原因。 首先，这将确定 α和β亚基突变体中的哪一个在 直接与其他蛋白质相互作用。 第二，识别 抑制子亚基将揭示蛋白质，所需的 将α和β亚基组装成F1寡聚体。

The H+ -ATPase of mitochondria, choloroplasts, and bacteria synthesizes ATP during respiration, providing the principal energy source for the cell. An important question in the field of Bioenergetics concerns the mechanism of this vitally important enzyme. The interactions within, and between, the F1 and F0 components that constitute the enzyme complex play an intimate role in the energy transduction reactions. The studies described on ATPase biogenesis address the fundamental question of how functionally important subunit contacts are initiated. The long-term goal of this project is to identify, and to define the function of, nuclear gene products required for biosynthesis of the mitochondrial F1-F0 complex in the yeast Saccharomyces cerevisiae. The basic objective is to provide information on the structure of the mitochondrial ATPase and to determine how the structure influences the function of the enzyme. The project is also aimed at answering the more global question of how macromolecular protein complexes are assembled from their component subunits. The present focus is specifically on assembly of the F1 component of the enzyme. Previous work with yeast mutants defective in F1 assembly has revealed a new class of proteins which are required for assembly of the F1 oligomer. An immediate goal is to purify these proteins and to use them in studies that will define their roles in F1 assembly. Extragenic revertants have already been isolated from yeast with mutations in the assembly factor genes. The suppressors will be cloned to identify proteins that interact directly with the F1 assembly factors. Strains are also available which are defective in F1 assembly due to mutations in the alpha and beta structural subunit genes. These mutants will be used to obtain extragenic revertants. The acquisition of these revertants will be important for two reasons. First, this will identify which of the alpha and beta subunit mutants are affected in domains that interact directly with other proteins. Second, identification of the suppressors subunits into will disclose proteins that are required for assembly of the alpha and beta subunits into the F1 oligomer.