GENETIC ANALYSIS OF LEARNING PERFORMANCE

学习表现的遗传分析

• 批准号: 2191395
• 负责人: BRIAN H. SMITH
• 金额: $ 8.89万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-05-01 至 1998-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2191395
• 关键词: Insecta; association learning; behavioral genetics; behavioral habituation /sensitization; discrimination learning; genetic markers; linkage mapping; male; molecular genetics; octopamine; olfactions; operant conditionings; performance; recombinant DNA; reversal learning; serotonin

Studies outlined below will investigate the quantitative and molecular genetic bases for variation in learning performance in the honey bee, Apis mellifera. Research into the bases of learning and memory in invertebrates such as mollusks and insects has demonstrated that fundamental properties of behavioral plasticity developed in studies of vertebrates apply across a broad phylogenetic spectrum. Yet, the genetic bases for learning traits are only beginning to be studied. Honey bees provide excellent opportunities for studying genetic factors that influence learning behavior because of the potential to apply several powerful behavioral paradigms for studying learning in vertebrates. The series of proposed studies will take advantage of the ability to train and analyze honey bee drones (males). Drones arise from unfertilized eggs and are thus haploid recombinants of the maternal genotype. Quantitative genetic selection on a haploid proceeds faster than it would on a diploid. Furthermore, linkage mapping from haploid genotypes using a map recently developed from a series of RAPD primers will not have the complications of such mapping using diploid genotypes. The specific aims of the studies are: * To further determine the quantitative genetic bases underlying variability in olfactory learning performance. Lines will be selected for fast versus slow reversal learning performance relative to unselected control lines. Use of this conditioning protocol and inclusion of appropriate control procedures will allow us to test whether associative learning performance can be selected without correlated responses in sensitization or motor systems. * To investigate the molecular genetic background using a linkage map established from RAPD genetic markers. These kinds of analyses will be carried out in recombinant F2 drone progeny of hybrid queens, in which spurious correlations due to founder effects in small base populations would be attenuated.

下面概述的研究将研究定量和分子方面的 蜜蜂学习成绩差异的遗传基础 西洋参。无脊椎动物学习记忆基础的研究 如软体动物和昆虫等已经显示出基本特性 在脊椎动物研究中发展出来的行为可塑性的 广泛的系统发育谱。然而，学习特征的遗传基础 才刚刚开始研究。蜜蜂提供了极好的 研究影响学习的遗传因素的机会 行为因为潜在地应用了几种强大的行为 脊椎动物学习研究的范例。一系列建议的 研究将利用训练和分析蜜蜂的能力 雄蜂(雄性)。雄蜂由未受精卵产生，因此是单倍体。 母系基因的重组体。水稻数量遗传选择研究进展 单倍体比二倍体进行得更快。此外，联动 利用新近开发的一种图谱从单倍体基因定位单倍体基因 一系列的随机扩增物不会有这种作图的复杂性 使用二倍体基因型别。这些研究的具体目的是： *进一步确定潜在的数量遗传基础 嗅觉学习成绩的变异性。将为以下项目选择行 相对于未选择的快反转学习性能与慢反转学习性能 控制线。该条件化方案的使用和纳入 适当的控制程序将使我们能够测试联合 可以在没有相关回答的情况下选择学习成绩 敏化或马达系统。 *利用连锁图谱调查分子遗传背景 用随机扩增多态性DNA遗传标记建立的。这些类型的分析将是 在杂交女王的重组F2雄蜂后代中进行，其中 小基数群体中创始人效应引起的虚假关联 会被衰减。