REGULATION OF PHOSPHOLIPID SYNTHESIS

磷脂合成的调控

基本信息

  • 批准号:
    2188655
  • 负责人:
  • 金额:
    $ 19.9万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1994
  • 资助国家:
    美国
  • 起止时间:
    1994-05-01 至 1998-04-30
  • 项目状态:
    已结题

项目摘要

In the yeast Saccharomyces cerevisiae, CTP is a precursor of all membrane phospholipids synthesized by the phosphatidylethanolamine methylation (primary) and CDP-ethanolamine- and CDP-choline-based (auxiliary) pathways. This grant application addresses the hypothesis that cellular levels of CTP regulate the synthesis of phospholipids by the primary and auxiliary pathways by controlling the expression and/or activities of the enzymes in these pathways. The effects of CTP on phospholipid synthesis will be examined in vivo using S. cerevisiae strains expressing various levels of CTP. CTP levels will be varied through the expression of the URA7 gene, which encodes CTP synthetase. The mRNA abundance and protein levels of phospholipid biosynthetic enzymes will be examined in response to various levels of CTP. Biochemical regulation of enzyme activities in response to CTP will also be examined. Since cellular CTP levels must be governed by the expression and/or activity of CTP synthetase. This application will also address if CTP synthetase activity is regulated by factors known to regulated phospholipid synthesis. CTP synthetase will be purified by molecular genetic and/or biochemical approaches for the purpose of preparing anti-CTP synthetase antibodies for regulation studies. The purified enzyme will be characterized with respect to its basic enzymological and kinetic properties. The regulation of CTP synthetase by cAMP-dependent protein kinase phosphorylation will be examined in vivo and in vitro. The information that will be obtained from these studies should be of great use to, and complement the work of other investigators in the field of phospholipid metabolism as well as those working on nucleotide metabolism.
在酿酒酵母中,CTP是所有膜的前体

项目成果

期刊论文数量(0)
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会议论文数量(0)
专利数量(0)

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GEORGE M. CARMAN其他文献

GEORGE M. CARMAN的其他文献

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{{ truncateString('GEORGE M. CARMAN', 18)}}的其他基金

Regulation and Role of Phosphatidate Phosphatase in Lipid Metabolism
磷脂酸磷酸酶在脂质代谢中的调节和作用
  • 批准号:
    10409651
  • 财政年份:
    2020
  • 资助金额:
    $ 19.9万
  • 项目类别:
Regulation and Role of Phosphatidate Phosphatase in Lipid Metabolism
磷脂酸磷酸酶在脂质代谢中的调节和作用
  • 批准号:
    9918539
  • 财政年份:
    2020
  • 资助金额:
    $ 19.9万
  • 项目类别:
Regulation and Role of Phosphatidate Phosphatase in Lipid Metabolism
磷脂酸磷酸酶在脂质代谢中的调节和作用
  • 批准号:
    10620311
  • 财政年份:
    2020
  • 资助金额:
    $ 19.9万
  • 项目类别:
Phospholipid metabolism and membrane function
磷脂代谢和膜功能
  • 批准号:
    8657370
  • 财政年份:
    2013
  • 资助金额:
    $ 19.9万
  • 项目类别:
Phospholipid Metabolism and Membrane Function
磷脂代谢和膜功能
  • 批准号:
    7889114
  • 财政年份:
    2009
  • 资助金额:
    $ 19.9万
  • 项目类别:
REGULATION OF PHOSPHOLIPID SYNTHESIS BY CTP
CTP 对磷脂合成的调控
  • 批准号:
    2415217
  • 财政年份:
    1994
  • 资助金额:
    $ 19.9万
  • 项目类别:
REGULATION OF PHOSPHOLIPID SYNTHESIS
磷脂合成的调控
  • 批准号:
    2188656
  • 财政年份:
    1994
  • 资助金额:
    $ 19.9万
  • 项目类别:
REGULATION OF PHOSPHOLIPID SYNTHESIS
磷脂合成的调控
  • 批准号:
    6918236
  • 财政年份:
    1994
  • 资助金额:
    $ 19.9万
  • 项目类别:
REGULATION OF PHOSPHOLIPID SYNTHESIS
磷脂合成的调控
  • 批准号:
    7418981
  • 财政年份:
    1994
  • 资助金额:
    $ 19.9万
  • 项目类别:
Regulation of Phospholipid Synthesis
磷脂合成的调节
  • 批准号:
    7646928
  • 财政年份:
    1994
  • 资助金额:
    $ 19.9万
  • 项目类别:
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