REGULATION OF PHOSPHOLIPID SYNTHESIS
磷脂合成的调控
基本信息
- 批准号:2188655
- 负责人:
- 金额:$ 19.9万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1994
- 资助国家:美国
- 起止时间:1994-05-01 至 1998-04-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In the yeast Saccharomyces cerevisiae, CTP is a precursor of all membrane
phospholipids synthesized by the phosphatidylethanolamine methylation
(primary) and CDP-ethanolamine- and CDP-choline-based (auxiliary)
pathways. This grant application addresses the hypothesis that cellular
levels of CTP regulate the synthesis of phospholipids by the primary and
auxiliary pathways by controlling the expression and/or activities of the
enzymes in these pathways. The effects of CTP on phospholipid synthesis
will be examined in vivo using S. cerevisiae strains expressing various
levels of CTP. CTP levels will be varied through the expression of the
URA7 gene, which encodes CTP synthetase. The mRNA abundance and protein
levels of phospholipid biosynthetic enzymes will be examined in response
to various levels of CTP. Biochemical regulation of enzyme activities in
response to CTP will also be examined. Since cellular CTP levels must be
governed by the expression and/or activity of CTP synthetase. This
application will also address if CTP synthetase activity is regulated by
factors known to regulated phospholipid synthesis. CTP synthetase will be
purified by molecular genetic and/or biochemical approaches for the
purpose of preparing anti-CTP synthetase antibodies for regulation
studies. The purified enzyme will be characterized with respect to its
basic enzymological and kinetic properties. The regulation of CTP
synthetase by cAMP-dependent protein kinase phosphorylation will be
examined in vivo and in vitro. The information that will be obtained from
these studies should be of great use to, and complement the work of other
investigators in the field of phospholipid metabolism as well as those
working on nucleotide metabolism.
在酿酒酵母中,CTP是所有膜的前体
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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GEORGE M. CARMAN其他文献
GEORGE M. CARMAN的其他文献
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{{ truncateString('GEORGE M. CARMAN', 18)}}的其他基金
Regulation and Role of Phosphatidate Phosphatase in Lipid Metabolism
磷脂酸磷酸酶在脂质代谢中的调节和作用
- 批准号:
10409651 - 财政年份:2020
- 资助金额:
$ 19.9万 - 项目类别:
Regulation and Role of Phosphatidate Phosphatase in Lipid Metabolism
磷脂酸磷酸酶在脂质代谢中的调节和作用
- 批准号:
9918539 - 财政年份:2020
- 资助金额:
$ 19.9万 - 项目类别:
Regulation and Role of Phosphatidate Phosphatase in Lipid Metabolism
磷脂酸磷酸酶在脂质代谢中的调节和作用
- 批准号:
10620311 - 财政年份:2020
- 资助金额:
$ 19.9万 - 项目类别: