ESCHERICHIA COLI 0157--H7 ADHERENCE AND COLONIZATION

大肠杆菌 0157--H7 粘附和定植

• 批准号: 2075462
• 负责人: PHILLIP I TARR
• 金额: $ 17.56万
• 依托单位: SEATTLE CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2075462
• 关键词: Escherichia coli; adhesin; antiserum; bacterial cytopathogenic effect; bacterial genetics; cell adhesion; clinical research; colitis; cow; gastrointestinal epithelium; gene deletion mutation; genetic transcription; host organism interaction; human subject; immunofluorescence technique; laboratory rabbit; phenotype; protein structure function; virulence

DESCRIPTION (Adapted from the applicant's abstract): Escherichia coli 0157:H7 is emerging as an important human gastrointestinal pathogen. This organism causes diarrhea, hemorrhagic colitis, and the hemolytic uremic syndrome (HUS). HUS is the most common cause of acute renal failure in childhood in North America, is increasing in incidence, and appears to be an important cause of chronic renal damage. The mechanism whereby E. coli 0157:H7 colonizes the gastrointestinal tracts of humans and cattle, its reservoir, is unknown. This project endeavors to identify and characterize the adhesin through which E. coli 0157:H7 adheres to epithelial cells, which will lead to a better understanding of the biology of gastrointestinal colonization by this pathogen. The long term objective of this project is the prevention or improved treatment of E. coli 0157:H7 infections. The PI will attempt to achieve this objective by preventing colonization or promoting clearance of E. coli 0157:H7 from mucosal surfaces of cattle and humans. To accomplish these objectives, they propose to (1) confirm that the molecule they have cloned and expressed is the adhesin of E. coli O157:H7, (2) study the role of this adhesin in the induction of colitis in rabbits, and in colonization of the gastrointestinal tract of cattle, (3) determine if this adhesin mediates attachment of E. coli 0157:H7 to human gastrointestinal tissue, and determine if infected humans develop antibodies to this molecule, and (4) study the molecular mechanisms underlying the expression of this adhesin in E. coli 0157:H7.

描述（改编自申请人的摘要）：大肠杆菌 0157：H7 正在成为一种重要的人类胃肠道病原体。 这种微生物会引起腹泻、出血性结肠炎和溶血性结肠炎。 尿毒症综合征（HUS）。 HUS 是急性肾病的最常见原因 在北美，儿童期失败的发生率正在增加，并且 似乎是慢性肾损伤的重要原因。 机制 大肠杆菌 0157:H7 定植于人类胃肠道 而作为其宿主的牛却是未知的。 该项目致力于通过以下方式识别和表征粘附素： 其中大肠杆菌 0157:H7 粘附在上皮细胞上，这将导致 更好地了解胃肠道定植的生物学 被这种病原体。 该项目的长期目标是 预防或改善大肠杆菌 0157:H7 感染的治疗。 PI 将试图通过防止殖民化或 促进牛粘膜表面大肠杆菌 0157:H7 的清除 和人类。 为了实现这些目标，他们建议 (1) 确认 他们克隆并表达的分子是大肠杆菌的粘附素。 大肠杆菌 O157:H7，(2) 研究这种粘附素在诱导中的作用 兔子结肠炎和胃肠道定植 牛，(3) 确定该粘附素是否介导大肠杆菌的附着 0157：H7进入人体胃肠组织，并判断是否感染 人类开发出针对该分子的抗体，并且（4）研究该分子 该粘附素在大肠杆菌 0157:H7 中表达的机制。