HOMEOBOXES IN XENOPUS DEVELOPMENT
非洲爪蟾发育中的同源盒
基本信息
- 批准号:2198294
- 负责人:
- 金额:$ 43.99万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1986
- 资助国家:美国
- 起止时间:1986-04-01 至 1996-03-31
- 项目状态:已结题
- 来源:
- 关键词:Xenopus alternatives to animals in research body regions congenital disorders developmental genetics embryogenesis endonuclease gene expression genetic manipulation genetic promoter element genetic regulation genetic transcription genetically modified animals homeobox genes in situ hybridization messenger RNA microinjections molecular cloning nucleic acid sequence regulatory gene transfection vertebrate embryology
项目摘要
The study of homeobox genes has substantially increased our understanding
of vertebrate embryogenesis. Work in the past Eve years has shown that
vertebrate homeodomain proteins, like their fruit fly counterparts, specify
the antero-posterior (A-P) identity of body regions. We now propose to
study how homeobox genes specify the gradual elaboration of the vertebrate
A-P axis.
We have recently found that microinjection of homeobox mRNA into
Xenopus laevis embryonic cells is sufficient to cause induction of new
axial structures after transplantation into a host embryo. This provides
a powerful direct method for testing whether different homeobox genes
confer regional specificity and, if so, which parts of the protein are
responsible. Other in vivo methods, such as microinjection of purified
mRNAs and antibodies, transplantation of germ layers, culture of explants,
and the generation of transgenic animals, will also be utilized.
Three crucial stages in amphibian development have been defined
by embryological studies. We propose to study Xenopus homeobox genes:
a) accumulated in the unfertilized egg
b) activated in the dorsal lip of the blastopore
c) expressed in the mesodermal mantle at the neurula stage.
By studying these genes, which act at different stages of the
same regulatory pathway, we hope to. understand how the vertebrate' body
plan is gradually established.
The proposed experiments exploit the accessibility of Xenopus
embryos to manipulation at stages before the aids is determined, and during
which equivalent experiments in mammalian embryos would be much more
difficult. However, because all vertebrates develop similarly, it is
expected that these studies will provide insights into the causes of
congenital malformation and embryo wastage in all vertebrates, including
man.
同源异型盒基因的研究大大增加了我们对
脊椎动物胚胎发生的过程 过去几年的工作表明,
脊椎动物的同源结构域蛋白,像果蝇的对应物一样,
身体区域的前后(A-P)一致性。 我们现建议
研究同源异型盒基因如何指定脊椎动物的逐渐发育
A-P轴
我们最近发现,将同源框mRNA显微注射到
非洲爪蟾胚胎细胞足以引起新的
移植到宿主胚胎后的轴向结构。 这提供
一种强有力的直接方法,
赋予区域特异性,如果是这样,蛋白质的哪些部分是
责任 其他体内方法,如显微注射纯化的
mRNA和抗体,胚层移植,外植体培养,
以及转基因动物的产生。
两栖动物发展的三个关键阶段已经被确定
胚胎学研究。 我们建议研究爪蟾同源盒基因:
a)在未受精卵中积累
B)在胚孔的背唇中激活
c)在神经胚阶段在中胚层套中表达。
通过研究这些基因,这些基因在不同的阶段发挥作用,
同样的调节途径,我们希望。了解脊椎动物的身体
计划正在逐步建立。
拟议的实验利用爪蟾的可及性
在艾滋病确定之前的阶段,以及
在哺乳动物胚胎中进行的同等实验
难 然而,由于所有脊椎动物的发育都是相似的,
预计这些研究将提供深入了解的原因,
所有脊椎动物的先天畸形和胚胎浪费,包括
伙计
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('EDWARD M DE ROBERTIS', 18)}}的其他基金
XENOPUS EGG LIGAND-ACTIVATED TRANSCRIPTION FACTORS
非洲爪蟾卵配体激活转录因子
- 批准号:
2200588 - 财政年份:1991
- 资助金额:
$ 43.99万 - 项目类别:
XENOPUS EGG LIGAND-ACTIVATED TRANSCRIPTION FACTORS
非洲爪蟾卵配体激活转录因子
- 批准号:
3329422 - 财政年份:1991
- 资助金额:
$ 43.99万 - 项目类别:
XENOPUS EGG LIGAND-ACTIVATED TRANSCRIPTION FACTORS
非洲爪蟾卵配体激活转录因子
- 批准号:
3329423 - 财政年份:1991
- 资助金额:
$ 43.99万 - 项目类别:
XENOPUS EGG LIGAND-ACTIVATED TRANSCRIPTION FACTORS
非洲爪蟾卵配体激活转录因子
- 批准号:
2200587 - 财政年份:1991
- 资助金额:
$ 43.99万 - 项目类别:
XENOPUS EGG LIGAND-ACTIVATED TRANSCRIPTION FACTORS
非洲爪蟾卵配体激活转录因子
- 批准号:
3329421 - 财政年份:1991
- 资助金额:
$ 43.99万 - 项目类别:
HOMEOBOX GENES AND THEIR TARGETS IN XENOPUS DEVELOPMENT
非洲爪蟾发育中的同源盒基因及其靶点
- 批准号:
7016372 - 财政年份:1986
- 资助金额:
$ 43.99万 - 项目类别: