ENZYMOLOGY

酶学

• 批准号: 5209098
• 负责人: RAYMOND BUDDE
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5209098
• 关键词: antineoplastics; cooperative study; drug design /synthesis /production; enzyme activity; enzyme inhibitors; molecular cloning; protein tyrosine kinase

This Laboratory Program represents the biochemistry section of a multidisciplinary effort to develop specific inhibitors against Src. This Program will be responsible for producing recombinant Src and other PTKs to (1) screen inhibitors, (2) determine the affinity of "hits" identified in the screen, (3) determine the specificity of the "hits" identified in the screen, and (4) perform structural studies of the Src active site. This laboratory program serves as a screen between the two synthetic chemistry laboratories and the two biological testing laboratories which functions to narrow down the thousands of compounds synthesized to those which are worth studying for biological efficacy. In addiction, it aids the synthetic chemistry sections in choosing which analogs to consider for synthesis and testing. The biochemistry lab has developed an infrastructure to produce recombinant PTKs and a high-through put screening of combinatorial libraries.

本实验室计划代表了一个生物化学部分， 多学科的努力，开发针对Src的特异性抑制剂。 这 项目将负责生产重组Src和其他PTK， (1)筛选抑制剂，（2）确定在 筛选，（3）确定在筛选中鉴定的“命中”的特异性， 筛选，和（4）进行Src活性位点的结构研究。 这 实验室程序充当两种合成化学之间的屏障 实验室和两个生物测试实验室，其功能是 将数千种合成的化合物缩小到 研究生物功效。 在成瘾中，它帮助合成 化学部分在选择哪些类似物考虑合成和 试验. 生物化学实验室已经开发出一种基础设施， 重组PTKs和高通量筛选组合 图书馆.