OPERATIONAL MECHANISMS OF AIRWAY RECEPTORS

拟议中的调查将直接进行 继续我们之前关于喉部和上呼吸道受体的工作， 以及相关的反射反应。根据先前对 水溶液对喉部感受器活性的影响，我们将研究 通过改变喉反射反应强度的可能性 粘膜表面液物理化学成分的变化 (目标1)。一种相对常见的疾病，胃食道反射，有 越来越多地被认为是糖尿病发生的病因。 喉部有许多病变。我们将研究盐酸胃酶的作用。 口服液应用于喉粘膜对神经功能的影响 器官：受体转导和相关的反射反应。组织学 将对喉粘膜和粘膜下层进行控制，以 描述损伤(炎症、坏死...)由于接触到 盐酸-胃蛋白酶溶液(目标2)。预计喉头是最大的 全呼吸道的反应性、致咳嗽部位和一些证据 支持这一预期。然而，相反的证据也是 可用。使用其中上呼吸道和下呼吸道 在功能隔离的情况下，我们将比较咳嗽和 其努力的强度与机械和化学刺激相当 喉部和气管支气管树。此外，我们还将评估 喉内肌及其颞肌的放电模式 喉声门下压力变化与声门下压的关系 气管支气管性咳嗽。我们亦会研究是否有可能更改 在气管、支气管性咳嗽中，喉部肌肉的分泌物模式， 在喉部去传入之后。发展方面的问题 喉部和气管、支气管对有害刺激的反应性 学习。

The investigations being proposed will be carried out in direct continuation of our previous work on laryngeal and upper airway receptors, and related reflex responses. On the basis of previous observations on the effect of water solutions on laryngeal receptors activity, we will examine the possibility of modifying the strength of laryngeal reflex responses by changes in the physico-chemical composition of the mucosal surface liquid (Aim #1). A relatively common disorder, gastroesophageal reflex, has increasingly been recognized as an etiologic factor int he development of many laryngeal lesions. We will examine the effects of a HCI-pepsin solution applied to the laryngeal mucosa on the neural function of this organ: receptor transduction and related reflex responses. Histological control of the laryngeal mucosa and submucosa will be carried out to characterize the damage (inflammation, necrosis...) due to the exposure to HCI-pepsin solutions (Aim #2). The larynx is expected to be the most reactive, tussigenic-site of the entire respiratory tract and some evidence favors this expectation. However, evidence to the contrary is also available. Using a preparation in which upper and lower airway are functionally isolated, we will compare the elicitability of cough and the intensity of its efforts to comparable mechanical and chemical irritation of the larynx and the tracheobronchial tree. Moreover, we will evaluate the pattern of discharge of intrinsic laryngeal muscles and their temporal relationship to changes in subglottic pressure in laryngeal and tracheobronchial cough. We will also study any possible alteration of laryngeal muscle pattern of discharge, in tracheobronchial-elicted cough, following deafferentation of the larynx. Developmental aspects of laryngeal and tracheobronchial reactivity to noxious stimuli will be studied.