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DIFFERENTIATION MARKERS OF ALVEOLAR EPITHELIAL CELLS

肺泡上皮细胞的分化标志物

基本信息

批准号：
2217647
负责人：
Jerome S Brody
金额：
$ 28.13万
依托单位：
BOSTON UNIVERSITY MEDICAL CAMPUS
依托单位国家：
美国
项目类别：
财政年份：
1985
资助国家：
美国
起止时间：
1985-07-15 至 1995-07-31
项目状态：
已结题

项目摘要

This competitive renewal application is concerned with the developmental biology of the pulmonary alveolus, specifically focusing on the regulation of differentiation of alveolar type 2 (T2) and type 1 (T1) cells. The differentiation of these cells is crucial to alveolar restructuring associated with lung development, postnatal lung growth and lung repair following injury. During the initial period of this grant we described membrane markers of T2 and T1 cell differentiation and developed several new cell culture systems in which expression of these markers could be studied. We have developed antibodies to several T2 and T1 cell membrane glycoproteins and have established T2 cell and whole lung cDNA expression libraries which we are now using to identify and clone the genes encoding for the differentiation-related proteins. We have developed preliminary data which has allowed us to formulate the hypothesis that is central to the present grant proposal. Alveolar epithelial cell differentiation involves both induction and repression of cell-specific genes. This process is regulated by inducible transcription factors which bind to specific consensus sequences of DNA with this protein-DNA interaction being regulated in part by methylation of the DNA binding sites. The experiments proposed in this renewal application define several genes associated with T2 and T1 cell differentiation, show that they are regulated in the epithelial cell systems which wee have described, and test the hypothesis that transcriptional factors and DNA methylation are important determinants of developmentally-regulated gene expression. In the process we will isolate the DNA-binding proteins that are responsible for inducing expression of the differentiation-related genes. We will also explore the function of the proteins encoded by these genes. The products of this research will be new insights into the control of alveolar epithelial cell differentiation and new information about specialized functions of T2 and T1 cells.

本竞争性续期申请与发展有关

项目成果

期刊论文数量（12）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Atrial natriuretic peptide modulates alveolar type 2 cell adenylyl and guanylyl cyclases and inhibits surfactant secretion.

心钠素调节肺泡 2 型细胞腺苷酸环化酶和鸟苷酸环化酶并抑制表面活性剂分泌。

DOI：
10.1016/s0167-4889(98)00023-8
发表时间：
1998
期刊：
Biochimica et biophysica acta
影响因子：
0
作者：
Panchenko,MP;Joyce-Brady,M;Starikova,MG;Oakes,SM;Adachi,R;Brody,JS;Dickey,BF
通讯作者：
Dickey,BF

[3H]thymidine incorporation does not correlate with growth state in cultured alveolar type II cells.

[3H]胸苷掺入与培养的 II 型肺泡细胞的生长状态无关。