REGULATION OF GABA-A RECEPTOR SUBUNIT EXPRESSION

GABA-A 受体亚基表达的调控

• 批准号: 2259657
• 负责人: DENNIS R GRAYSON
• 金额: $ 6.45万
• 依托单位: ALLEGHENY-SINGER RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2259657
• 关键词: GABA receptor; cell differentiation; developmental neurobiology; embryo /fetus cell /tissue; granule cell; in situ hybridization; laboratory rat; polymerase chain reaction; receptor expression; tissue /cell culture

In the context of the research proposal, a competitive PCR assay will be used to analyze the profiles of expression of the mRNAs encoding subunits of the GABA-A receptor during development (in vivo) and as cerebellar granule cells differentiate in vitro. This information will be correlated with a comparable in situ hybridization analysis during development in vivo. The hypothesis that cell type specific patterns of expression of the receptor subunit mRNAs are influenced by both heterologous and homologous receptor stimulation will be tested. In the context of, the mechanism by which this trans-synaptic regulation of GABA-A receptor subunit mRNAs occurs will be investigated.

在研究提案的背景下，将采用竞争性PCR测定法， 用于分析编码亚基的mRNA的表达谱 GABA-A受体在发育过程中（体内）和小脑 颗粒细胞在体外分化。这些信息将与 在开发过程中进行了可比较的原位杂交分析， vivo. 细胞类型特异性表达模式的假设， 受体亚基mRNA受异源和 将测试同源受体刺激。在这方面， GABA-A受体的跨突触调节机制 将研究亚基mRNA的发生。