DNA SEQUENCING BY LASER-BASED SPECTROMETRY

这份提案概述了一个计划，重点是使用最先进的 解决重要生物学问题的实验方法。这个 RCDA提案的近期目标是发展一种新的高速 DNA测序仪。该仪器的实现将使DNA 以接近每百万个碱基的速率进行测序 一天的速度比目前的速度快三个数量级 技巧。为了实现这一目标，目前的DNA测序策略 将与三项新技术相结合，生产出一种低成本、易操作的 自动测序方法。使用激光汽化来转移标签 DNA链进入气相。一旦进入气相共振- 增强的多光子电离被用来精确地放置一个单位的 每条链上的正电荷，然后飞行时间质量分析 已执行。提议的技术的自动化将导致 极其灵敏和经济高效的DNA测序方法需要 体力劳动很少。

This proposal outlines a program focused on using state-of-the-art experimental methods to address important biological problems. The immediate goal of this RCDA proposal is the development of a new high speed DNA sequencer. The realization of this instrument will allow DNA sequencing to be performed at a rate approaching one million bases per day - a rate three orders of magnitude faster than currently available techniques. To achieve this objective current DNA sequencing strategies will be combined with three new technologies to produce a low-cost, easily automated sequencing method. Laser vaporization is used to transfer tagged strands of DNA into the gas phase. Once in the gas phase resonance- enhanced, multiphoton ionization is used to place precisely one unit of positive charge on each strand and then time-of-flight mass analysis performed. The automation of the technology proposed would result in an extremely sensitive and cost effective DNA sequencing method requiring little manual labor.