DNA SEQUENCING BY LASER-BASED SPECTROMETRY

该提案概述了一个专注于使用最先进的 解决重要生物学问题的实验方法。 的 该RCDA提案的直接目标是开发一种新的高速 DNA测序仪 这个仪器的实现将使DNA 测序将以接近100万个碱基/ 天--比目前可用的速率快三个数量级 技术. 为了实现这一目标，目前的DNA测序策略 将与三种新技术相结合， 自动测序方法。 激光汽化用于转移标记的 DNA链进入气相。 一旦进入气相共振- 增强的多光子电离被用来精确地放置一个单位的 每条链上带正电荷，然后进行飞行时间质量分析 执行。 拟议的技术自动化将导致 非常灵敏和成本有效的DNA测序方法， 一点点体力劳动。

This proposal outlines a program focused on using state-of-the-art experimental methods to address important biological problems. The immediate goal of this RCDA proposal is the development of a new high speed DNA sequencer. The realization of this instrument will allow DNA sequencing to be performed at a rate approaching one million bases per day - a rate three orders of magnitude faster than currently available techniques. To achieve this objective current DNA sequencing strategies will be combined with three new technologies to produce a low-cost, easily automated sequencing method. Laser vaporization is used to transfer tagged strands of DNA into the gas phase. Once in the gas phase resonance- enhanced, multiphoton ionization is used to place precisely one unit of positive charge on each strand and then time-of-flight mass analysis performed. The automation of the technology proposed would result in an extremely sensitive and cost effective DNA sequencing method requiring little manual labor.