ROLE AND REGULATION OF PLATELET PROTEIN KINASE C
血小板蛋白激酶 C 的作用和调节
基本信息
- 批准号:2221138
- 负责人:
- 金额:$ 20.63万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1990
- 资助国家:美国
- 起止时间:1990-07-01 至 1995-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Protein kinase C (PKC) has emerged as a critical enzyme in signal
transduction, cell regulation, cell differentiation, and tumor promotion.
The enzyme is now known to exist as a family of closely-related isoforms
whose individual mechanism and function has not been defined. In human
platelets, PKC has been implicated in a number of platelet functions
including secretion and aggregation as well as negative feedback mechanisms
(on platelet activation). We have identified four isoenzymes of PKC in
platelets. The long-term goals are to define the role of PKC in platelet
function. This proposal aims at studying the hypothesis that different PKC
isoenzymes are differentially regulated to transduce selective functions.
Our laboratory, which has extensive experience in the biochemical
characterization of PKC and in the study of platelet biology, is uniquely
suited to evaluate these questions. The specific aims of this proposal are,
therefore, directed at determining the mechanism and significance of PKC
isoenzymes from platelets. These will be addressed by: 1) purifying PKC
isoenzymes from platelets (using FPLC) and studying their in vitro
regulation (using mixed micellar methodologies that we have developed); 2)
determining the intracellular localization of PKC isoenzymes in resting and
activated platelets, respectively, (by monitoring enzyme activity, phorbol
binding and by Western blots) and; 3) studying the in situ (physiologic)
regulation of platelet PKC isoenzymes and determining their selective
substrate phosphorylations (using an in vitro model that we have
developed). These studies will provide the necessary biochemical background
for determining the mechanism and physiologic regulation of PKC isoenzymes.
Such knowledge is of great significance for improving our understanding of
the role of platelets in hemostasis and in the pathophysiology of
arteriovascular disease. This improved biochemical knowledge may lead to
more efficacious and rational antiplatelet drug development. Knowledge
gained from the platelet model should prove to be of great usefulness for
investigators studying the regulation and role of PKC isoenzymes in various
other cell systems.
蛋白激酶C (Protein kinase C, PKC)已成为信号处理的关键酶
项目成果
期刊论文数量(0)
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会议论文数量(0)
专利数量(0)
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{{ truncateString('YUSUF AWNI HANNUN', 18)}}的其他基金
Protein kinase C in Lung Cancer with mutant EGFR
EGFR 突变肺癌中的蛋白激酶 C
- 批准号:
10618917 - 财政年份:2020
- 资助金额:
$ 20.63万 - 项目类别:
Protein kinase C in Lung Cancer with mutant EGFR
EGFR 突变肺癌中的蛋白激酶 C
- 批准号:
10454776 - 财政年份:2020
- 资助金额:
$ 20.63万 - 项目类别:
Protein kinase C in Lung Cancer with mutant EGFR
EGFR 突变肺癌中的蛋白激酶 C
- 批准号:
9888660 - 财政年份:2020
- 资助金额:
$ 20.63万 - 项目类别:
Neutral Sphingomyelinases and Bioactive Ceramides
中性鞘磷脂酶和生物活性神经酰胺
- 批准号:
10437811 - 财政年份:2016
- 资助金额:
$ 20.63万 - 项目类别:
Neutral Sphingomyelinases and Bioactive Ceramides
中性鞘磷脂酶和生物活性神经酰胺
- 批准号:
10640899 - 财政年份:2016
- 资助金额:
$ 20.63万 - 项目类别:
Neutral sphingomyelinases and bioactive ceramides
中性鞘磷脂酶和生物活性神经酰胺
- 批准号:
9071506 - 财政年份:2016
- 资助金额:
$ 20.63万 - 项目类别:
Diversity Supplement for Bernandie Jean: Neutral sphingomyelinases and bioactive ceramides
Bernandie Jean 的多样性补充:中性鞘磷脂酶和生物活性神经酰胺
- 批准号:
9752140 - 财政年份:2016
- 资助金额:
$ 20.63万 - 项目类别: