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REGULATION OF MUCIN GENE EXPRESSION

REGULATION OF MUCIN GENE EXPRESSION
粘蛋白基因表达的调控
批准号：
2221166
负责人：
CAROL B BASBAUM
金额：
$14.87万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
1990
资助国家：
美国
项目状态：
已结题
起止时间：
1990-07-01 至 1995-07-31
来源：
https://reporter.nih.gov/project-details/2221166
关键词：
cell type complementary DNA deoxyribonuclease I gene expression genetic library genetic transcription laboratory rat molecular cloning mucins nucleic acid sequence nucleoproteins polymerase chain reaction respiratory airway clearance respiratory epithelium transcription factor transfection western blottings

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英文摘要
(Adapted from the investigator's specific aims) The specific aims of this revised application are: 1) Isolate full length mucin cDNAs from rat and human airway libraries; from these determine the primary structure of mucin core protein in both species. 2) Using the rat mucin cDNA, isolate the cognate gene from a rat genomic library and identify upstream (or other) regulatory elements using transfection analysis of deletion mutants. 3) Determine the extent to which these regulatory elements bind nuclear proteins using mobility shift and dNase I protection assays and 4) Establish a rat model of respiratory mucin overproduction and determine whether transcriptional control of mucin biosynthesis is upregulated using a rat mucin cDNA in northern hybridization and nuclear run-on assays.
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