LUNG MACROPHAGE MOTILITY--ROLE OF FIBRONECTIN RECEPTORS

越来越多的证据表明肺泡巨噬细胞的缺陷 功能可能与观察到的肺机会主义有关 HIV-1感染者的感染情况。该受赠人将审查 纤维连接蛋白受体在控制黏附和运动中的作用 肺泡巨噬细胞的特性。在鉴定纤维连接蛋白之后 肺巨噬细胞上的受体(整合素)与纤维连接蛋白结合， 调查人员计划使用一个数学模型来指导他们检查 受体-配体(纤维连接蛋白)相互作用在调节细胞周期中的作用 肺泡巨噬细胞的黏附强度。这种互动的作用 在调节巨噬细胞的运动行为方面，将会被确定。 最后，调查人员将使用视频显微镜和图像分析 来测试他们关于细胞速度和细胞速度之间关系的假设 纤维连接蛋白密度。理解这些相互作用可能会有广泛的影响 对其他受体配体相互作用的适用性，并可提供 对细胞表面受体相互作用的重要认识 以及与细胞外基质中的结构结合的配体。

Accumulating evidence suggests that defects in alveolar macrophage function may contribute to the observed pulmonary opportunistic infections in HIV-1 infected individuals. This grantee will examine the role of fibronectin receptors in controlling the adherence and motile properties of alveolar macrophages. After identifying fibronectin receptors (integrins) on lung macrophages that bind to fibronectin, the investigators plan to use a mathematical model to guide them in examining the role of receptor-ligand (fibronectin) interactions in regulating the adhesive strength of alveolar macrophages. The role of this interaction in regulating the motile behavior of macrophages will then be determined. Finally the investigators will employ video microscopy and image analysis to test their hypotheses regarding relationships between cell speed and fibronectin density. Understanding these interactions could have broad applicability to other receptor ligand interactions and could provide important understanding of the interaction between cell surface receptors and ligands bound to structures in the extracellular matrix.