LUNG MACROPHAGE MOTILITY--ROLE OF FIBRONECTIN RECEPTORS

肺巨噬细胞活力——纤连蛋白受体的作用

• 批准号: 2771356
• 负责人: RONALD P DANIELE
• 金额: $ 28.05万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-30 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2771356
• 关键词: alveolar macrophages; cell adhesion; cell motility; fibronectins; flow cytometry; fluid flow; human subject; image processing; immunoprecipitation; integrins; mathematical model; mechanical stress; model design /development; monoclonal antibody; receptor; receptor expression; video microscopy

Accumulating evidence suggests that defects in alveolar macrophage function may contribute to the observed pulmonary opportunistic infections in HIV-1 infected individuals. This grantee will examine the role of fibronectin receptors in controlling the adherence and motile properties of alveolar macrophages. After identifying fibronectin receptors (integrins) on lung macrophages that bind to fibronectin, the investigators plan to use a mathematical model to guide them in examining the role of receptor-ligand (fibronectin) interactions in regulating the adhesive strength of alveolar macrophages. The role of this interaction in regulating the motile behavior of macrophages will then be determined. Finally the investigators will employ video microscopy and image analysis to test their hypotheses regarding relationships between cell speed and fibronectin density. Understanding these interactions could have broad applicability to other receptor ligand interactions and could provide important understanding of the interaction between cell surface receptors and ligands bound to structures in the extracellular matrix.

越来越多的证据表明肺泡巨噬细胞的缺陷 功能可能有助于观察到的肺机会性 HIV-1感染者的感染。 该承授人将审查 纤维连接蛋白受体在控制粘附和运动中的作用 肺泡巨噬细胞的特性。 鉴定纤连蛋白后 受体（整合素）的肺巨噬细胞结合纤连蛋白， 研究人员计划使用一个数学模型来指导他们检查 受体-配体（纤连蛋白）相互作用在调节 肺泡巨噬细胞的粘附强度。 这种相互作用的作用 调节巨噬细胞运动行为的作用。 最后，研究人员将使用视频显微镜和图像分析 为了测试他们关于细胞速度和 纤连蛋白密度。 了解这些相互作用可以有广泛的 适用于其他受体配体相互作用，并可提供 对细胞表面受体之间相互作用的重要理解 和与细胞外基质中的结构结合的配体。