CORE--EXPOSURE AND ANALYTICAL FACILITY

曝光和分析核心作为所有人的资源 本计划项目内的调查人员，并作为服务的渠道 生物医学和生态学之间的联系。 该核心提供 主要有三个方面：（1）。动物处理和暴露，这 包括哺乳动物、无脊椎动物和水生生物; 2.）化学 分析和样品制备，包括组织中毒物的测定， 血液沉淀物和水; 3.）动物细胞和组织制备， 包括同质细胞群的分离、手术和尸检。 该核心通过提供以下内容，为生物标志物开发提供了统一的重点 暴露于相同条件下的动物的细胞和组织制备物 生物医学组件的条件。 这使得直接 正在开发的生物标志物测定法的有效性比较。 此外，该核心还开展了旨在提供 关于毒物对生物医学的生物利用度的信息 件. 这些示范项目是在合作中进行的 与生态部门的成员合作，他们提供以下方面的专业知识 暴露途径、相关剂量和药代动力学分析。 因此 核心计划利用生态学各组成部分提供的数据， 生物利用度信息的生物医学组件。 以这种方式 Core将生态学组件的发现整合到研究中 由生物医学组件进行。 在过去，核心进行了一次 不同器官中铬清除率的药代动力学分析 慢性暴露于铬酸盐后 在下一个赠款期间， 将进行两项验证性研究，一项是关于 另一个是与食物中的蛋白质结合的镉， 肺暴露于铬酸盐后铬的生物利用度- 被污染的土壤 这两项研究都将在 生态学成分、细胞和组织样本的研究人员 将被送到生物医学中心的研究者手中。 件. 因此，核心小组的这些活动将有助于将 生态和生物医学组件的专业知识，并提供服务， 两者

The Exposure and Analytical Core functions as a resource for all investigators within this Program Project and acts as a conduit of services between the Biomedical and Ecological components. This Core provides services in three main areas: 1.) animal handling and exposure, this includes mammals, invertebrates and aquatic organisms; 2.) chemical analysis and sample preparation, including assays of toxicants in tissue, blood sediment, and water; 3.) animal cell and tissue preparation, including isolation of homogeneous cell populations, surgery and necropsy. This Core furnishes a unifying focus for biomarker development by providing cell and tissue preparations from animals exposed under identical conditions to the Biomedical components. This allows for direct comparisons of the effectiveness of the biomarker assays under development. In addition, this Core conducts investigatory projects designed to provide information concerning the bioavailability of toxicants to the Biomedical components. These investigatory projects are performed in collaboration with members of the Ecology components who provide expertise regarding routes of exposure, relevant dosages and pharmacokinetic analysis. Thus the Core uses data provided by the Ecology components to furnish bioavailability information to the Biomedical components. In this way the Core integrates the findings of the Ecology components into the studies conducted by the Biomedical components. In the past, the Core conducted a pharmacokinetic analysis of the clearance of chromium from various organs after chronic exposure to chromate. In the coming grant period, the Core will conduct two investigatory studies, one concerns the bioavailability of ingested cadmium bound to protein in food while the other concerns the bioavailability of chromium after pulmonary exposure to chromate- contaminated soil. Both of these studies will be conducted with investigators of the Ecology components, and the cell and tissue samples from exposed animals will be delivered to investigators in the Biomedical components. Thus, these activities of the Core will serve to integrate the expertise of the Ecology and Biomedical components and provide services to both.