CORE--EXPOSURE AND ANALYTICAL FACILITY

曝光和分析核心对所有人来说都是一个资源 本计划项目中的调查人员，并充当服务的渠道 在生物医学和生态组成部分之间。此核心提供 三个主要领域的服务：1.)动物的处理和暴露，这个 包括哺乳动物、无脊椎动物和水生生物；化学 分析和样品准备，包括组织中毒物的分析， 血沉淀物和水；3)动物细胞和组织制剂， 包括同质细胞群体的分离、手术和尸检。 该核心为生物标记物的开发提供了统一的重点，通过提供 暴露在相同环境下的动物的细胞和组织制剂 生物医学组件的条件。这允许直接 正在开发中的生物标记物分析的有效性比较。 此外，该中心开展调查性项目，旨在提供 有关毒物生物利用度的信息 组件。这些调查项目是在合作中进行的 与生态部门的成员一起提供关于以下方面的专业知识 暴露途径、相关剂量及药代动力学分析。因此， 核心使用生态组件提供的数据来提供 生物医学组件的生物利用度信息。通过这种方式， CORE将生态组成部分的研究结果纳入研究 由生物医学组件进行。在过去，核心进行了一项 铬在不同器官中清除的药代动力学分析 在长期接触铬酸盐之后。在即将到来的授权期内，核心 将进行两项调查性研究，一项是关于生物利用度的 摄入的镉与食物中的蛋白质结合，而另一个关注的是 铬在肺部接触铬酸盐后的生物可利用性 被污染的土壤。这两项研究都将在 生态成分、细胞和组织样本的研究人员 将被送到生物医学杂志的研究人员手中 组件。因此，核心的这些活动将有助于将 生态和生物医学组件的专业知识，并提供服务 两者都有。

The Exposure and Analytical Core functions as a resource for all investigators within this Program Project and acts as a conduit of services between the Biomedical and Ecological components. This Core provides services in three main areas: 1.) animal handling and exposure, this includes mammals, invertebrates and aquatic organisms; 2.) chemical analysis and sample preparation, including assays of toxicants in tissue, blood sediment, and water; 3.) animal cell and tissue preparation, including isolation of homogeneous cell populations, surgery and necropsy. This Core furnishes a unifying focus for biomarker development by providing cell and tissue preparations from animals exposed under identical conditions to the Biomedical components. This allows for direct comparisons of the effectiveness of the biomarker assays under development. In addition, this Core conducts investigatory projects designed to provide information concerning the bioavailability of toxicants to the Biomedical components. These investigatory projects are performed in collaboration with members of the Ecology components who provide expertise regarding routes of exposure, relevant dosages and pharmacokinetic analysis. Thus the Core uses data provided by the Ecology components to furnish bioavailability information to the Biomedical components. In this way the Core integrates the findings of the Ecology components into the studies conducted by the Biomedical components. In the past, the Core conducted a pharmacokinetic analysis of the clearance of chromium from various organs after chronic exposure to chromate. In the coming grant period, the Core will conduct two investigatory studies, one concerns the bioavailability of ingested cadmium bound to protein in food while the other concerns the bioavailability of chromium after pulmonary exposure to chromate- contaminated soil. Both of these studies will be conducted with investigators of the Ecology components, and the cell and tissue samples from exposed animals will be delivered to investigators in the Biomedical components. Thus, these activities of the Core will serve to integrate the expertise of the Ecology and Biomedical components and provide services to both.