PILOT STUDY--PAIRED HELICAL FILAMENT BINDING PROTEINS

试点研究——配对螺旋丝结合蛋白

• 批准号: 3790275
• 负责人: GEORGE PERRY
• 金额: --
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3790275
• 关键词: autoradiography; binding proteins; cow; cytoskeleton; electron microscopy; hippocampus; human tissue; laboratory rat; microfilaments; microtubule associated protein; microtubules; neurofibrillary tangles; neurofilament; paired helical filament; polymerization; tau proteins

Although many studies have addressed the issue of the cellular components present in PHF none has defined the relationship of these components to PHF structure nor to each other. This lack of knowledge has resulted from a focus on components rather than their relationship. Further it has suffered from the necessity of a hypothesis with regard to likely components before a putative PHF component is examined. In the present proposal, an in vitro model system is developed utilizing the properties of polymers to define the relationship of identified PHF components as well as opening the possibility for identification of new PHF components. The utility of this approach is the possibility of assigning PHF components to a minimum of two classes, core or associated proteins, by non-immunochemical methods. PHF components detected by this protocol, but not identifiable by immunochemical approaches, will be defined by amino acid sequencing. These studies will provide an under standing of PHF as polymers current to that known for the most prevalent cellular linear polymer, the cytoskeleton.

尽管许多研究已经解决了细胞的问题 PHF中存在的组件没有定义 这些成分既不到PHF结构，也不到彼此。这种缺乏 知识源于对组件的关注，而不是其 两性关系。此外，它还受到了一种 关于推定的PHF之前的可能成分的假设 组件进行了检查。在本提案中，体外模型 系统是利用聚合物的性质开发的，以定义 已鉴定的PHF组分与开度的关系 鉴定新的PHF组分的可能性。这个 这种方法的用途是将PHF分配给 组件至少包含两个类，即核心类或关联类 蛋白质，通过非免疫化学方法。检测到PHF组件 通过这个方案，但不能用免疫化学方法识别 方法，将通过氨基酸测序来确定。这些 研究将提供对PHF作为聚合物电流的理解 与已知的最流行的细胞线性聚合物相比， 细胞骨架。