PILOT STUDY--PAIRED HELICAL FILAMENT BINDING PROTEINS

虽然许多研究已经解决了细胞的问题， PHF中存在的组件没有定义 这些组件与PHF结构也不相互关联。 这种缺乏 知识来自于对组件的关注，而不是它们的 关系 此外，它还遭受了一个 关于假定PHF前可能成分的假设 组件进行了检查。 在本提议中，体外模型 系统开发利用聚合物的性质，以定义 识别的PHF组件之间的关系以及开口 识别新的PHF组件的可能性。 的 这种方法的效用是可以分配PHF 组件至少分为两类，核心或关联 蛋白质，通过非免疫化学方法。 检测到PHF成分 通过该方案，但通过免疫化学法无法识别 方法，将通过氨基酸测序来定义。 这些 研究将提供一个理解PHF作为聚合物电流 对于已知的最普遍的多孔线性聚合物， 细胞骨架

Although many studies have addressed the issue of the cellular components present in PHF none has defined the relationship of these components to PHF structure nor to each other. This lack of knowledge has resulted from a focus on components rather than their relationship. Further it has suffered from the necessity of a hypothesis with regard to likely components before a putative PHF component is examined. In the present proposal, an in vitro model system is developed utilizing the properties of polymers to define the relationship of identified PHF components as well as opening the possibility for identification of new PHF components. The utility of this approach is the possibility of assigning PHF components to a minimum of two classes, core or associated proteins, by non-immunochemical methods. PHF components detected by this protocol, but not identifiable by immunochemical approaches, will be defined by amino acid sequencing. These studies will provide an under standing of PHF as polymers current to that known for the most prevalent cellular linear polymer, the cytoskeleton.