ROLE OF RAS-RELATED RABI IN VESICULAR TRANSPORT

RAS 相关 RABI 在水泡运输中的作用

• 批准号: 2085165
• 负责人: ELLEN J TISDALE
• 金额: $ 3.12万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-03-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2085165
• 关键词: Golgi apparatus; SDS polyacrylamide gel electrophoresis; Vesiculovirus; complementary DNA; endoplasmic reticulum; gene mutation; guanine nucleotide binding protein; hydrolysis; immunofluorescence technique; intracellular transport; molecular cloning; molecular genetics; monoclonal antibody; point mutation; posttranslational modifications; protein structure function; protein transport; recombinant DNA; site directed mutagenesis; tissue /cell culture; transfection; vaccinia virus

Although the secretory pathway is required for the survival of the individual cell and for the organism as a whole, the mechanism which governs vesicular transport is poorly understood. Recent data have shown that the rab family of ras-related small GTP-binding proteins are involved in the regulation of membrane traffic between distinct subcellular compartments. The long-term objective of this fellowship proposal is to characterize the role of the rab 1 gene product in protein export from the endoplasmic reticulum (ER) to the Golgi complex. It is only through the knowledge of normal cellular function that one can begin to understand where a defect could lead to a disease process. Structural information available for the ras protein has made it possible to map functional domains within the ras primary sequence. Site-directed mutations in the rab 1 gene analogous to known functional domains of ras will be generated. These mutated forms of rab (either encoded within a plasmid or a recombinant vaccinia virus) will be introduced into tissue culture cells, then evaluated for their effect on protein trafficking, in vivo. In addition, an in vitro transport assay developed in this laboratory which reconstitutes transport between the ER and cis Golgi compartments will be employed to study the biochemical function of rab 1 and to identify and isolate upstream or downstream effector molecules which interact with rab 1 and are required in the secretory pathway.

尽管分泌途径是细胞存活所必需的