C JUN, C FOS AND NEURONAL PROGRAMMED CELL DEATH

程序性细胞死亡(PCD)是公认的发育现象 这是通过细胞凋亡的过程来实现的。最近的证据有力地表明 PCD也是导致病理状况的因素。几行 有证据表明，PCD是由特定基因的表达引起的 基因程序导致蛋白质杀死细胞。我们已经确定了 交感神经元中表达增加的几个基因 在剥夺神经营养因子NGF的情况下进行PCD， 以及许多表达减少的基因。我们已经提交了证据 C-jun，一个由其中一个诱导基因编码的转录因子， 是神经元性PCD所必需的。我们还提出了证据，证明 涉及Fos家族是细胞凋亡所必需的；在缺乏NGF的情况下 神经元，某些Fos家族成员，即c-fos和fos B被诱导， 而其他潜在的抑制性家族成员，即FRA-1和FRA-2， 都被压制了。这些结果使我们推测神经元性PCD依赖于 根据某些基因的激活，即c-jun，和抑制 其他基因，即fra-1和fra-2。由于(I)c-jun的诱导是 最早可检测到的遗传事件，(Ii)c-jun与每个Fos相互作用 家庭成员，和(Iii)c-jun被明确牵连为 死亡是必要的，我们目前的工作假设是 诱导c-jun，调节c-jun的活性 C-jun的作用，构成生化和遗传学的关键部分 导致神经元死亡的级联反应。我们建议进行有针对性的攻击 来评估这一假说。 我们将进一步定义Jun和Fos家庭成员在 使用几种分子遗传学方法的神经元死亡。我们会 分析这些基因在蛋白质水平上的表达，以进行研究 可能的转录后调控。我们将研究一下 蛋白质：Jun和Fos家族成员之间的蛋白质相互作用 直接鉴定与c-jun相互作用的蛋白质。我们会 评估保护神经元免受NGF剥夺的药物，即， CAMP类似物和钾去极化，通过激活CREB-2，a 这些试剂激活并调节c-jun的转录因子。 我们将确定细胞凋亡过程中的生化变化，这取决于 C-jun，以确定c-jun行动的“下游”机制。我们将 确定导致激活的分子和细胞机制 C-Jun；这项调查将在时间级联中引导 通过评估氧化应激和c-jun的作用来评估事件 磷酸化。 这些研究将对JUN的作用提供相当大的洞察力 Fos家族成员在神经元性PCD中的作用 神经元性PCD。鉴于越来越多的证据表明神经元性PCD在 神经系统的病理状态，即中风、神经毒性、 和神经退行性疾病，这些见解可能会导致策略 药物干预以防止或延缓这些情况下的死亡。

Programmed cell death (PCD) is a well-recognized developmental phenomenon that occurs by a process of apoptosis. Recent evidence strongly suggests that PCD also contributes to pathological conditions. Several lines of evidence have indicated that PCD results from the expression of a specific genetic program leading to proteins that kill the cell. We have identified several genes whose expression in increased in sympathetic neurons undergoing PCD in response to deprivation of the neurotrophic factor, NGF, and many genes whose expression is decreased. We have presented evidence that c-Jun, a transcription factor encoded by the one of the induced genes, is required for neuronal PCD. We have also presented evidence that implicate the Fos family as being necessary for apoptosis; in NGF-deprived neurons, certain Fos family members, i.e., c-fos and fos B, are induced, while other, potentially inhibitory family members, i.e., fra-1 and fra-2, are repressed. These results led us to theorize that neuronal PCD depends upon both the activation of certain genes, i.e., c-jun, and the repression of other genes, i.e., fra-1 and fra-2. Since (i) the induction f c-jun is the earliest detectable genetic event, (ii) c-Jun interacts with each Fos family member, and (iii) c-Jun has been implicated specifically as necessary for death, our current working hypothesis is that events that induce c-jun, modulate the activity of c-Jun, and that emanate from the actions of c-Jun, constitute critical portions of a biochemical and genetic cascade that results in neuronal death. We propose a focused attack aimed at evaluating this hypothesis. We shall further define the roles of the jun and Fos family members in neuronal death by using several molecular genetic approaches. We shall analyze athe expression of these genes at athe protein level to investigate possible post-transcriptional regulation. We shall examine the protein:protein interactions among the Jun and Fos family members to identify directly athe proteins that interact with c-Jun. We shall evaluate whether agents that protect neurons from NGF deprivation, i.e., cAMP analogs and potassium depolarization, do so by activating CREB-2, a transcription factor that these agents activate and that modulates c-Jun. We shall determine the biochemical changes during apoptosis that depend on c-Jun, to identify 'downstream" mechanisms of c-Jun action. We shall identify the molecular and cellular mechanisms leading to the activation of c-jun; this investigation will lead "upstream" in the temporal cascade of events by evaluating the roles of oxidative stress and c-Jun phosphorylation. These studies will provide considerable insight into the role of the Jun and Fos family members in neuronal PCD and athe overall mechanism of neuronal PCD. Given the increasing evidence for a role of neuronal PCD in pathological conditions of the nervous system, i.e., stroke, neurotoxicity, and neurodegenerative disease, these insights may lead to strategies to intervene pharmacologically to prevent or retard death in these conditions.