MOUSE AND RAT CHROMOSOME SPECIFIC DNAS AND LIBRARIES

描述(改编自申请者的摘要)：特定于染色体 所有小鼠和大鼠染色体的DNA都将通过结合 染色体显微切割和简并寡核苷酸技术 引物聚合酶链式反应(DOP-PCR)扩增。流排序的困难 染色体阻止了一整套染色体特有的DNA或 人类以外的图书馆不能使用。显微解剖 将绕过这些困难，并允许纯染色体专一性 从所有的染色体中提取DNA。一种单一的简并引物 将允许从小鼠和大鼠身上扩增DNA。COT1 DNA用于 在杂交中用作竞争者将为两个物种做好准备。 染色体特异的DNA将通过绘画来测试其特异性。 选定的DNA将通过杂交到一个 一组独特的染色体特异探针。染色体特有的DNA 将使用常规技术进行克隆。染色体特有的DNA， COT1 DNA，以及使用的方案将可供研究使用 社区。

DESCRIPTION (Adapted from applicant's abstract): Chromosome-specific DNAs will be prepared for all mouse and rat chromosomes by combining the technologies of chromosome microdissection and degenerate oligonucleotide primed PCR (DOP-PCR) amplification. Difficulties with flow sorting of chromosomes have prevented complete sets of chromosome-specific DNAs or libraries other than human from being made available. Microdissection will circumvent these difficulties and allow pure chromosome-specific DNAs to be prepared from all chromosomes. A single degenerate primer will allow amplification of DNA from both mouse and rat. Cot1 DNA for use as competitor in hybridizations will be prepared for both species. Chromosome-specific DNAs will be tested for specificity by painting. Selected DNAs will be tested for representation by hybridization to a battery of unique chromosome-specific probes. Chromosome-specific DNAs will be cloned using conventional techniques. Chromosome-specific DNAs, Cot1 DNA, and protocols for use will be made available to the research community.