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MOUSE CHROMOSOME-SPECIFIC PAINTING PROBES

MOUSE CHROMOSOME-SPECIFIC PAINTING PROBES
小鼠染色体特异性绘画探针
批准号：
3499068
负责人：
MELISSA C LIECHTY
金额：
$4.99万
依托单位：
APPLIED GENETICS LABORATORIES, INC.
依托单位国家：
美国
项目类别：
财政年份：
1993
资助国家：
美国
项目状态：
已结题
起止时间：
1993-08-16 至 1994-02-15
来源：
https://reporter.nih.gov/project-details/3499068
关键词：
genetic mapping in situ hybridization method development nucleic acid probes polymerase chain reaction

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中文摘要
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英文摘要
This proposal describes a new technology called "preparative in situ hybridization (Prep-I.S.H.), which is capable of isolating a set of cDNA or genomic clones according to their chromosome location. The technology takes advantage of in situ hybridization of cloned DNAs to metaphase chromosome spreads, chromosome microdissection, and the polymerase chain reaction (PCR). We propose to use this technology to develop a set of chromosome-specific painting probes for the mouse. The demonstration case, a chromosome 11-specific probe, will be used to paint the mitotic chromosome 11s derived from the mouse lymphoma assay (MLA).
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PREPARATION OF USER FRIENDLY MOUSE CHROMOSOME PAINT KITS
PREPARATION OF USER FRIENDLY MOUSE CHROMOSOME PAINT KITS
PREPARATION OF USER FRIENDLY MOUSE CHROMOSOME PAINT KITS
MOUSE AND RAT CHROMOSOME SPECIFIC DNAS AND LIBRARIES
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