NOVEL SURFACES BY PLASMA TECHNIQUE FOR DNA DETECTION

描述(摘自申请者的摘要)：等离子体技术 聚合将在二期工程中使用，以修改表面 转印膜和吸墨膜。这项研究有可能 提高DNA检测的灵敏度和多功能性，特别是 用化学发光法。这项工作可能会带来更广泛的选择 更换面临日益严重的环境和环境污染的放射性探头 监管机构的反对。 根据第一阶段的结果，对普通转印/印迹的修改 通过等离子体过程(主要是等离子体聚合物沉积)获得的膜 预计将使敏感度提高四到十倍。一个 用于30厘米宽乘以1000的连续治疗的原型等离子系统 正在开发的是一米长的膜材。具体目标 是：等离子体工艺的优化；对影响的扩展研究 膜上DNA、RNA和蛋白质结合的修饰 杂交；膜表面形态与 非同位素检测中的生物化学功效.确认 商用探头系统与优化的膜处理兼容。

DESCRIPTION (Adapted from applicant's abstract): The technique of plasma polymerization is to be used in a Phase II project to modify the surfaces of transfer and blotting membranes. The research has the potential to increase the sensitivity and versatility of DNA detection, particularly with chemiluminescent methods. The work may lead to wider options for replacement of radioactive probes that face growing environmental and regulatory opposition. Based on the Phase I results, modification of common transfer/blotting membranes by plasma processes (primarily plasma polymer deposition) is expected to give a four to tenfold improvement in sensitivity. A prototype plasma system for continuous treatment of 30-cm wide by 1000 meter long membrane stock is to be developed. The specific objectives are: optimization of plasma processes; extended study of the effects of modification on binding of DNA, RNA, and proteins in membrane hybridization; correlation of membrane surface morphology with biochemical efficacy in non-isotopic detection; confirmation of commercial probe systems compatible with optimized membrane treatments.