NOVEL SURFACES BY PLASMA TECHNIQUE FOR DNA DETECTION

用于 DNA 检测的等离子技术的新颖表面

• 批准号: 2209813
• 负责人: HIROSHI NOMURA
• 金额: $ 20.36万
• 依托单位: NEOMECS
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1997-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2209813
• 关键词: artificial membranes; biomedical equipment development; chemical binding; method development; nucleic acid hybridization; nucleic acid probes; plasma; polymerization

DESCRIPTION (Adapted from applicant's abstract): The technique of plasma polymerization is to be used in a Phase II project to modify the surfaces of transfer and blotting membranes. The research has the potential to increase the sensitivity and versatility of DNA detection, particularly with chemiluminescent methods. The work may lead to wider options for replacement of radioactive probes that face growing environmental and regulatory opposition. Based on the Phase I results, modification of common transfer/blotting membranes by plasma processes (primarily plasma polymer deposition) is expected to give a four to tenfold improvement in sensitivity. A prototype plasma system for continuous treatment of 30-cm wide by 1000 meter long membrane stock is to be developed. The specific objectives are: optimization of plasma processes; extended study of the effects of modification on binding of DNA, RNA, and proteins in membrane hybridization; correlation of membrane surface morphology with biochemical efficacy in non-isotopic detection; confirmation of commercial probe systems compatible with optimized membrane treatments.

描述（改编自申请人摘要）：血浆技术 聚合将用于第二阶段项目，以修改表面 转移和印迹膜。这项研究有可能 提高DNA检测的灵敏度和通用性，特别是 用荧光的方法。这项工作可能会导致更广泛的选择， 更换放射性探针， 监管反对。 基于I期结果，修改了常见的转移/印迹 通过等离子体工艺（主要是等离子体聚合物沉积）的膜是 预期灵敏度提高四到十倍。一 原型等离子体系统，用于连续处理30厘米宽× 1000 米长的膜股票将被开发。的具体目标 是：等离子体工艺的优化;效果的扩展研究 对膜上DNA、RNA和蛋白质结合的修饰 杂交;膜表面形态与 非同位素检测的生化功效; 与优化的膜处理相容的商业探针系统。