NOVEL SURFACES BY PLASMA TECHNIQUE FOR DNA DETECTION

用于 DNA 检测的等离子技术的新颖表面

• 批准号: 3498814
• 负责人: HIROSHI NOMURA
• 金额: $ 5万
• 依托单位: NEOMECS
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3498814
• 关键词: DNA; alkenes; bioluminescence; chemical cleavage; fluorocarbon polymers; hydropathy; immunologic assay /test; luminescence; membrane; method development; monomer; oxygen; photosensitizing agents; plastics; polymerization; polymers; pyrenes; surface property; ultraviolet radiation

DESCRIPTION (Adapted from applicant's abstract):The proposed research has the long-term goal of increasing the sensitivity and range of application of clinical chemiluminenscent immunoassays. Substantial public benefits could follow. Specific objectives: (1) Prepare preliminary assay substrates by plasma surface modification. (2) Test substrates for background luminescence and non-specific protein binding. (3) Prepare additional substrates using designated plasma monomers. (4) Test substrates for background luminescence and chemiluminescence levels in immunoassays. (5) Analyze results and describe their relevance to a Phase II program leading to expanded commercial applications of chemiluminescent assay.

描述（改编自申请人的摘要）：拟议的研究具有 提高灵敏度和应用范围的长期目标 临床荧光免疫分析。 实质性公共利益 可以遵循。 具体目标：（1）准备初步分析 通过等离子体表面改性的基材。 (2)测试基板 背景发光和非特异性蛋白结合。 (3)准备 使用指定的等离子体单体的附加衬底。 (4)测试 背景发光和化学发光水平的底物 免疫测定。 (5)分析结果并描述其与阶段的相关性 II计划导致扩大商业应用的荧光 比色法